BIOPHYSICAL CHANGES IN CELL MEMBRANES DURING APOPTOSIS & CELLULAR TRAUMA

细胞凋亡过程中细胞膜的生物物理变化

• 批准号: 8170956
• 负责人: JOHN BELL
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170956
• 关键词: Apoptosis; Binding; Calcium; Cell Death; Cell membrane; Cells; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Eukaryotic Cell; Fluorescence; Funding; Grant; Hydrolysis; Institution; Lateral; Lipid Bilayers; Measurement; Membrane; Microscopy; Phospholipase A2; Phospholipids; Property; Research; Research Personnel; Resources; Source; Spatial Distribution; Techniques; Trauma; United States National Institutes of Health; Work; enzyme activity; laurdan; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Secretory phospholipase A2 (sPLA2) binds to lipid bilayers and catalyzes hydrolysis of phospholipids. Normally, cells resist the enzyme's action, but they become susceptible during apoptosis or trauma. The focus of our research group is to understand what physical changes occur in the membranes of eukaryotic cells when they are traumatized by elevated intracellular calcium or induced into apoptosis by various means. The purpose is to understand the relationship between membrane structure and cell death and between membrane structure and the action of phospholipase A2. The fluorescence of membrane probes such as laurdan and patman are studied by two-photon microscopy to separate changes occuring at the plasma membrane from those that pertain to internal membranes. Moreover, these techniques allow us to identify the lateral spatial distribution of membrane properties and their relationship to apoptosis and enzyme activity. In the past, the work has been confined to static measurements. Plans are in place to begin using FCS to assess potential changes in the dynamics of membrane properties during apoptosis and trauma.

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