CT PERFUSION IMAGING USING IODINATED LIPOSOMES

使用碘化脂质体进行 CT 灌注成像

• 批准号: 7601143
• 负责人: SRINIVASAN MUKUNDAN
• 金额: $ 1.5万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601143
• 关键词: Affinity; Blood; Blood - brain barrier anatomy; Blood Circulation; Blood Volume; Cerebrum; Characteristics; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Contrast Media; Encapsulated; Engineering; Extravasation; Funding; Goals; Grant; Half-Life; Hour; Image; Injection of therapeutic agent; Institution; Liposomes; Measurement; Measures; Microscopic; Perfusion; Permeability; Phospholipids; Polyethylene Glycols; Rate; Relative (related person); Research; Research Personnel; Resources; Source; Standards of Weights and Measures; Surface; Time; United States National Institutes of Health; Vesicle; novel; research study; size; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary goal of this proposal is to overcome the confounding effects of a leaky blood brain barrier in tumors by using a novel CT contrast agent, iodinated liposome. Liposomes are microscopic spherical vesicles constructed of phospholipid bilayers that can encapsulate the iodinated contrast agent. Vesicle size, chemical affinity, and thermal (or pH) sensitivities can be engineered. We will use polyethylene glycol (PEG)-coated liposome with an intravascular circulation half-life of 6-8 hours. These characteristics allow blood pool imaging for several hours after a simple IV injection, and should also decrease the rate of extravasation of the agent into the tumor interstitium. The relative enhancement of a voxel on a CT study is proportional to the intravoxel concentration of contrast agent. Agents that behave as true bloodpool agents on the time scale of this experiment should remain within the intravascular space. Therefore, relative enhancement of a voxel should be proportional to the cerebral blood volume (CBV). This should allow for direct measurement of CBV, rather than the need for using a deconvolution approach. Moreover, reinjection with a standard iodinated contrast agent can be performed soon afterward, and a more accurate permeability surface product determination can be obtained because CBV has been accurately measured.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该提案的主要目标是通过使用新型 CT 造影剂碘化脂质体来克服肿瘤中血脑屏障渗漏的混杂效应。脂质体是由磷脂双层构成的微小球形囊泡，可以封装碘化造影剂。囊泡大小、化学亲和力和热（或 pH）敏感性可以进行设计。我们将使用聚乙二醇 (PEG) 包被的脂质体，其血管内循环半衰期为 6-8 小时。这些特性允许在简单的静脉注射后几个小时内进行血池成像，并且还应该降低药剂外渗到肿瘤间质中的速率。 CT 研究中体素的相对增强与造影剂的体素内浓度成正比。在该实验的时间尺度上表现得像真正的血池药剂的药剂应该保留在血管内空间内。因此，体素的相对增强应与脑血量（CBV）成正比。 这应该允许直接测量 CBV，而不需要使用反卷积方法。此外，随后可以立即重新注射标准碘造影剂，并且由于CBV已被准确测量，因此可以获得更准确的渗透性表面积测定。