FOURIER DOMAIN LOW COHERENCE INTERFEROMETRIC MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们研制了一种傅里叶域反射相位成像显微镜。 一个共同的路径相位参考检测方案允许在kHz带宽的细胞位移的埃尺度测量。 可以通过扫描样品或光束来执行二维成像。利用该系统，我们获得了单个培养的HEK 293细胞和神经元的高分辨率反射相位图像。 测试对象的成像演示了仪器的亚纳米稳定性和对细胞运动的敏感性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a Fourier domain reflectance phase imaging microscope. A common-path phase-referenced detection scheme allows angstrom-scale measurements of cell displacements at kHz bandwidths. Two-dimensional imaging can be performed by scanning the sample or the beam. Using this system, we have obtained high resolution reflection phase images of single cultured HEK293 cells and neurons. Imaging of test objects demonstrates the instruments sub-nanometer stability and sensitivity to cell motions.