FOURIER DOMAIN LOW COHERENCE INTERFEROMETRIC MICROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们研制了一台傅里叶域反射位相成像显微镜。公共路径相位基准检测方案允许以埃尺度测量千赫带宽上的单元位移。可以通过扫描样品或光束来执行二维成像。利用该系统，我们获得了单个培养的HEK293细胞和神经元的高分辨率反射相图像。对测试对象的成像显示了该仪器亚纳米级的稳定性和对细胞运动的敏感性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a Fourier domain reflectance phase imaging microscope. A common-path phase-referenced detection scheme allows angstrom-scale measurements of cell displacements at kHz bandwidths. Two-dimensional imaging can be performed by scanning the sample or the beam. Using this system, we have obtained high resolution reflection phase images of single cultured HEK293 cells and neurons. Imaging of test objects demonstrates the instruments sub-nanometer stability and sensitivity to cell motions.