ELECTROSTATIC PROPERTIES OF CCMV AND CMV

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 病毒衣壳或蛋白质壳的组装和拆解是病毒生命周期中必不可少的步骤。对病毒组装/拆解的了解可以为其他寡聚过程提供额外的见解，例如通过类似的机制发生的大型细胞组装中的蛋白质结合。此外，仅了解衣壳的性质和功能对于推断包膜遗传物质进入和释放宿主细胞所涉及的所有步骤都很重要，并有助于提出通过基于结构的抗病毒治疗药物设计来干扰衣壳组装和稳定性的有效方法。 豌豆褪绿斑驳病毒(CCMV)是研究最深入的病毒之一，是一种正链RNA植物侵染病毒，属于布罗夫病科。它由蛋白质衣壳和病毒RNA组成，病毒RNA被包装在里面。由于其二十面体结构和组装所需的基因产物数量有限，它已被广泛用作理解生物组装的模型系统。CCMV为理论和实验研究提供了一个可访问的模型系统，用于检查调节病毒组装、拆解和稳定性的过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Assembly and disassembly of a viral capsid, or a protein shell, are essential steps in the life cycle of a virus. Understanding of viral assembly/disassembly can provide additional insights to other oligomerization processes as in protein association in large cellular assemblies, which occurs through a similar mechanism. Additionally, understanding the properties and function of the capsid shell alone is important for inferring all steps involved in host cell entry and release of the enveloped genetic material and can aid in proposing efficient ways of interfering with capsid assembly and stability via structure-based design of antiviral therapeutics. One of the most intensely studied viruses has been the cowpea chlorotic mottle virus (CCMV), a positive strand RNA plant-infecting virus which belongs to the Bromoviridae family. It is composed of a protein capsid and viral RNA which is packed inside. It has been widely used as a model system for understanding of biological assembly because of its icosahedral structure and the limited number of gene products required for its assembly. CCMV offers an accessible model system for examining the processes that regulate viral assembly, disassembly, and stability, both for theoretical and experimental studies.