FRET ANALYSIS OF FISSION YEAST SPB COMPONENTS

裂殖酵母 SPB 成分的 FRET 分析

• 批准号: 7602234
• 负责人: Kathleen L Gould
• 金额: $ 0.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602234
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Fission Yeast; Fluorescence Resonance Energy Transfer; Funding; Grant; Institution; Microtubule-Organizing Center; Proteins; Research; Research Personnel; Resources; Signal Transduction; Source; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to gain further understanding of the fission yeast SPB, its organization must be determined. Beginning with Ppc89, we plan to build a picture of how this microtubule organizing center is assembled. We already have evidence for direct interaction between Ppc89 and another SPB protein involved in assembling a signaling cascade, Sid4, and now plan to move forward with other sets of FRET partners using these two proteins as vertices.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了进一步了解裂解酵母SPB，必须确定其组织结构。从PPC89开始，我们计划建立一个关于这个微管组织中心是如何组装的图景。我们已经有证据表明Ppc89和另一种参与组装信号级联反应的SPB蛋白Sid4之间存在直接相互作用，现在我们计划利用这两种蛋白作为顶点，与其他组FRET合作伙伴进行合作。