MOLECULAR LOGIC OF SUBSTRATE RECOGNITION BY THE CULLIN-4 UBIQUITIN LIGASE

CULLIN-4 泛素连接酶识别底物的分子逻辑

• 批准号: 7602224
• 负责人: NING ZHENG
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602224
• 关键词: Affinity Chromatography; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Cullin Proteins; Family; Funding; Grant; Human; Institution; Logic; Mass Spectrum Analysis; Molecular; Proteins; Recruitment Activity; Research; Research Personnel; Resources; Source; Ubiquitination; United States National Institutes of Health; base; receptor; ubiquitin ligase; ubiquitin-protein ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cullin based ubiquitin ligases are the largest family of E3 ligases. There is 7 Cullins in humans and every Cullin utilizes a different family of substrate specific receptors in order to recruit the substrate to the ubiquitination machinery. Although the family of substrate specific receptors are known for most of the Cullins, the identity of this family for the Cullin-4 family remains elusive. Using tandem-affinity purification of Cullin-4 and DDB1 protein complexes from human cells and mass spectrometry we will attempt to 1) identify and 2) characterize the family of substrate-specirfic receptors for Cullin-4.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 基于Cullin的泛素连接酶是E3连接酶的最大家族。 人类有7个Cullin，每个Cullin都利用一个 不同的底物特异性受体家族，以便将底物募集到泛素化机器。 虽然 底物特异性受体家族对于大多数Cullin是已知的，该家族对于Cullin-4家族的同一性 仍然难以捉摸 使用串联亲和纯化来自人细胞和团块的Cullin-4和DDB 1蛋白复合物 通过质谱分析，我们将尝试1）鉴定和2）表征Cullin-4的底物特异性受体家族。