TRANSLOCATION OF INHALED ULTRAFINE ELEMENTAL CARBON PARTICLS TO EXTRAPUL TISSUE

吸入的超细元素碳颗粒转移至超薄组织

• 批准号: 7602410
• 负责人: GUNTER OBERDORSTER
• 金额: $ 2.1万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602410
• 关键词: Adverse effects; Air; Animals; Brain; Breathing; Caliber; Carbon; Cerebellum; Cerebrum; Chemicals; Clinical Research; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Deposition; Epidemiologic Studies; Funding; Gases; Grant; Heart; In Vitro; Injection of therapeutic agent; Institution; Kidney; Label; Liver; Long-Term Effects; Lung; Medical; Methods; Nanotechnology; Organ; Particulate; Pollution; Range; Rattus; Research; Research Personnel; Resources; Respiratory System; Site; Source; Spleen; Tissues; Ultrafine; United States National Institutes of Health; accelerator mass spectrometry; anthropogenesis; base; particle; size; ultrafine particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ultrafine particles (particles 100 nm in diameter) are ubiquitous in the ambient air from anthropogenic and natural sources (e.g., internal combustion engine emissions, gas to particle conversions). The new field of nanotechnology in industrial and medical use adds an additional component. Inhalation of ultrafine particles is known to cause adverse effects in pulmonary and extrapulmonary tissues. Based on our earlier studies with ultrafine 13C elemental carbon particles we hypothesize that such particles can translocate from sites of deposition to extrapulmonary organs such as the liver and the brain. Due to the inherent problem of 13C background levels in tissues for assessing significant increases in added tissue carbon a more sensitive method is needed. We propose to generate ultrafine elemental carbon particles labeled with 14C to which rats will be exposed by inhalation, gavage and i.v. injection to these particles. The size of the ultrafine 14C particles ranges from ~10 nm to ~80 nm. Rats will be sacrificed at different timepoints after exposure and lungs, liver, heart, kidney, spleen and brain sections (olfactory; cerebrum; cerebellum) excised and prepared for 14C accelerator mass spectrometry. These studies are part of an EPA-funded Ultrafine Particle Center at Rochester. Studies performed by Center investigators focus on physico-chemical characterization of ambient UFP; epidemiological studies; controlled clinical studies; toxicological animal studies; and mechanistic in vitro studies. The proposed research in collaboration with LLNL will have great significance to elucidate the fate of inhaled ultrafine particles after their deposition in the respiratory tract. If significant amounts of ultrafine particles are translocated to sensitive sites such as the heart and the brain, it may explain both short-term and long-term effects of ambient particulate pollution.

这个子项目是众多研究子项目之一