COBRE: LSU: MOLECUL NEUROBIOL CORE RESOURCE MODULE: TRANSGENIC KNOCKOUT ANIMALS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子神经生物学核心模块(MNCM)由Rene Anand博士和Erickson博士指导。两个研究伙伴提供MNCM的服务。MNCM允许研究人员使用Biorad的福陆-S MAX MultiImager系统，通过荧光或化学发光检测方法对样品进行检测，包括凝胶、斑点和缩微板，从而获得高质量的图像。该设施配备了一台安捷伦科技公司的生物分析器RNA纯度扫描仪。该设施提供从细胞和组织中提取和纯化RNA的指导。该核心也是与基因芯片分析核心的联系，基因芯片分析核心包含Affymetrix 428阵列扫描仪和417阵列，用于筛选DNA微阵列。MNCM还为基因分型和维持科布雷提案过程中使用的转基因和敲除动物的库存所需的其他专门护理提供了高质量的共享资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Neurobiology Core Module (MNCM) is directed by Dr. Rene Anand and Dr. Erickson. Two Research Associates provide the services of the MNCM. The MNCM allows investigators to obtain high-quality images produced by fluorescence or chemiluminescence detection methods of samples including gels, blots, and microplates, using the Fluor-S MAX MultiImager System from BioRAD. This facility houses a Bioanalyzer Agilent Technologies RNA purity scanner. The facility provides guidance about extracting and purifying RNA from cells and tissues. This core is also a linkage with the gene chip analysis core that houses the Affymetrix 428 Array Scanner and 417 Arrayer for screening DNA microarrays. The MNCM also provides a high-quality shared resource for genotyping and other specialized care needed for maintaining stocks of transgenic and knock-out animals used during the course of the COBRE proposal.