Protein Core

蛋白质核心

• 批准号: 7667780
• 负责人: John Atkinson
• 金额: $ 19.9万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7667780
• 关键词: B-Lymphocytes; Commercial Sources; Core Protein; Disease model; Funding; Generations; Hybridomas; Laboratories; Monoclonal Antibodies; Operative Surgical Procedures; Production; Proteins; Reagent; Recombinant Fusion Proteins; Recombinant Proteins; Research; Research Personnel; Rheumatism; Role; Services; Technology; Testing; Training; biomedical scientist; cost; interest; protein function; scale up; success

To analyze a protein of interest, it is necessary to develop reagents that can detect it. Furthermore, to investigate the role of a protein in rheumatic disease models, it is frequently desirable to use monoclonal antibodies (mAbs) or recombinant proteins that block the function of the protein in question. A Protein Core has been established and has been productive during the last funding period. The RDCC proposes to continue support of this facility to provide services through a 1) Hybridoma facility to produce new mAbs; and 2) Production and Purification facility to produce high quality purified mAbs or recombinant proteins of interest. New services are planned to enhance the facility. Thus, the facility will continue to provide investigators with large quantities of proteins that can be used to analyze their molecules in relationship to rheumatic disease models.

为了分析感兴趣的蛋白质，有必要开发可以检测它的试剂。 为了研究蛋白质在风湿性疾病模型中的作用，经常需要使用单克隆抗体， 抗体（mAb）或重组蛋白，其阻断所述蛋白的功能。蛋白质核心 在上一个供资期间，已经建立并取得了成效。协调委员会建议 继续支持该设施，通过1）杂交瘤设施提供服务，以生产新的mAb;以及 2)生产和纯化设施，用于生产高质量的纯化mAb或重组蛋白， 兴趣计划提供新的服务以加强该设施。因此，该设施将继续提供 研究人员拥有大量的蛋白质，可以用来分析它们的分子与 风湿病模型。