Protein Core

蛋白质核心

• 批准号: 8379367
• 负责人: John Atkinson
• 金额: $ 18.26万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8379367
• 关键词: Communities; Core Facility; Core Protein; Generations; Growth; Hybridomas; Immunology; Monoclonal Antibodies; Oryctolagus cuniculus; Production; Proteins; Recombinants; Research; Research Personnel; Rheumatism; Services; System; Testing; Universities; Washington; genetic immunization strategies; member

The Protein Core facility is one of the research cores in the Rheumatic Diseases Core Center (RDCC) at Washington University. It proposes the following Specific Aims to sustain and develop new state-of-the-art protein services to RDCC members: 1) Support continued progress and growth of the Hybridoma Center; and 2) Support continued progress and growth of the Protein Production and Purification Facility. The Hybridoma Center aids RDCC investigators in the generation of primary hybridomas secreting monoclonal antibodies that are critical to contemporary immunology and rheumatic diseases research. New services are being tested to offer RDCC investigators rabbit monoclonal antibodies and genetic immunization, for example. The Protein Production and Purification Facility aids RDCC investigators by producing and purifying large quantities of monoclonal antibodies for their studies. In addition, it has recently gained expertise in producing and purifying recombinant and native proteins from a number of different expression systems. This service will now be made available to the RDCC community.

蛋白质核心设施是华盛顿大学风湿病核心中心（RDCC）的研究核心之一。它提出了以下具体目标，以维持和发展新的最先进的蛋白质服务RDCC成员：1）支持杂交中心的持续进步和增长; 2）支持蛋白质生产和纯化设施的持续进步和增长。杂交瘤中心帮助RDCC研究人员产生分泌单克隆抗体的原代杂交瘤，这些单克隆抗体对当代免疫学和风湿性疾病研究至关重要。例如，正在测试新的服务，为RDCC调查人员提供兔单克隆抗体和遗传免疫。蛋白质生产和纯化设施通过生产和纯化大量用于研究的单克隆抗体来帮助RDCC研究人员。此外，它最近获得了从许多不同表达系统中生产和纯化重组和天然蛋白质的专业知识。这项服务现在将提供给RDCC社区。