Histology Core

肌肉骨骼组织学核心 形态技术被生物学家广泛使用，并越来越多地被用作 生物医学工程师的结果衡量标准，特别是在组织工程方面。因此，许多 研究基地的调查人员(例如，Apte、Ballock、Muschler、Bauer、Midura、Lefebvre、 德温，麦克德维特)从事一系列组织学技术，包括组织化学 冰冻、石蜡和塑料包埋组织的染色，脱钙和 未脱钙组织、组织阵列的生成、免疫组织化学和原位 杂交。目前，这些调查人员大多已经建立了这样或那样的证据， 但不是所有的，组织学方法在自己的实验室里或有限地使用组织学 核心服务位于机构(LRI)成像核心。我们建议设立一个 肌肉骨骼组织学核心，将提供处理所需的特定专业知识 肌肉骨骼组织以及研究基地更好地利用常规和 特殊染色、免疫组织化学、不脱钙组织分析和原位杂交。 该核心将为常规组织学服务和自动化提供完整的服务 免疫组织化学以及研究人员培训和参与专门方法 例如原位杂交。拟议的核心利用了现有的机构核心 SERVICE在软组织常规组织学方面的专业知识，并在 肌肉骨骼组织送到研究基地。肌肉骨骼组织学的可用性 进一步加强研究基地利用现有资源的能力 成像核心，并可以提供在机器人中进行的研究的形态关联 和机械测试核心。

Musculoskeletal Histology Core Morphological techniques are widely used by biologists and are being increasingly used as outcome measures by biomedical engineers, especially in tissue engineering. Accordingly, many investigators in the Research Base (e.g., Apte, Ballock, Muschler, Bauer, Midura, Lefebvre, Derwin, McDevitt) undertake a range of histological techniques, including histochemical staining of frozen, paraffin and plastic-embedded tissue, analysis of decalcified and undecalcified tissue, generation of tissue arrays, immunohistochemistry and in situ hybridization. At the present time, most of these investigators have established one or another, but not all, histological methods in their own laboratories or make limited use of the histology core service located in the institutional (LRI) imaging core. We propose to establish a Musculoskeletal Histology Core that will provide the specific expertise required for handling musculoskeletal tissues as well as improved access of the Research Base to routine and specialized stains, immunohistochemistry, undecalcified tissue analysis and in situ hybridization. The core will provide complete service for routine histological services and automated immunohistochemistry as well as investigator training and participation in specialized methods such as in situ hybridization. The proposed core leverages the existing institutional core service's expertise in routine histology of soft tissue and provides special expertise in musculoskeletal tissue to the Research Base. The availability of musculoskeletal histology further enhances the ability of the Research Base to exploit the resources available through the Imaging Core and could provide morphological correlates of studies undertaken in the Robotics and Mechanical Testing Core.