Microarray

微阵列

基本信息

批准号：
7630235
负责人：
ALBERT DE LA CHAPELLE
金额：
$ 22.89万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7630235
关键词：
Algorithms Analysis of Variance Archives Arts Behavior Bioinformatics Biological Assay Biophysics Biostatistics Shared Resource Budgets Canada Cancer Center Support Grant Capillary Electrophoresis Charge Clinical Clinical Research Cluster Analysis Collaborations Color Complementary DNA Computer Workstations Computer software Condition Consultations Controlled Study Custom DNA Data Data Analyses Data Storage and Retrieval Databases Disease Doctor of Philosophy Ensure Equipment Evaluation Experimental Designs Faculty Fee-for-Service Plans Floor Funding Gene Expression Genes Genetic Genetic Programming Genome Genotype Germany Goals Grant Graph Hand Histocompatibility Testing Hour Housekeeping Gene Human Resources Image Image Analysis Information Storage Institution Journals Label Laboratories Linear Models Malignant Neoplasms Manufacturer Name Measurement Measures Medical Methods Microarray Analysis Microarray Shared Resource Modeling Molecular Biology Molecular Profiling Mutation Nature Nebraska Neuro-Oncological Ventral Antigen 2 New Mexico New York North Carolina Nucleotides Numbers Ohio Oligonucleotides Patients Polymerase Chain Reaction Portugal Price Procedures Process Production Protocols documentation Publications Quality Control RNA RNA Processing RNA analysis Randomized Rate Recommendation Regression Analysis Relative (related person)Reliability of Results Reproducibility Research Research Infrastructure Research Personnel Resource Sharing Reverse Transcriptase Polymerase Chain Reaction Running Sampling Scanning Scheme Science Sensory Services Site Standards of Weights and Measures Structure Sum Surveys Switzerland System Technology Testing The Comprehensive Cancer Center Arthur G James Cancer Hospital and Richard J Solove Research Institute at Ohio State University Time Tissue-Specific Gene Expression Transcript Universities Validation Washington Week Wilcoxon Rank Test Work anticancer research base beta Actin biomedical informatics cancer genetics cost data mining day design improved interest investigator training member posters preference professor programs research facility research study statistics tool

项目摘要

Parallel analysis of multiple genes on the DNA or RNA level is becoming more and more important, because cancer is identified as a disease that involves multiple genes. To study this cascade of genetic alterations, analysis on a single-gene level cannot be the final answer. Microarray analysis is state-of-the-art technology for fulfilling these scientific needs. The OSUCCC Microarray Shared Resource (MASR) was established as a "developing" Shared Resource in 1998 initially serving investigators in the Molecular Biology and Cancer Genetics Program with genome wide expression analysis, and subsequently becoming one of the Shared Resources for the entire OSUCCC offering genome wide expression analysis using Affymetrix GeneChips. In September 2002, quantification of RNA integrity by capillary electrophoresis became available, and since September 2003, the MASR has offered validation of microarray results, via an array-based design of primers for real-time PCR. For projects utilizing any of these technologies, the MASR offers multiple services, including experimental design consultation, sample processing, hybridization, scanning, full statistical analysis and consultation, and validation of results. Newer services, such as custom arrays and SNPs are soon to be available. The MASR trains investigators to upload their results to Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/) since this becomes mandatory for publication in many journals. The MASR website (www.dnaarrays.org) contains information about services, publications and downloadable protocols and order forms. The MASR offers timely, technical and professional expertise that promotes high quality science at a competitive price. To support OSUCCC researchers, several hundred RNA samples are processed each year for genome wide expression analysis. In addition, several hundred RNA samples are processed for integrity quantification. During 2003, the MASR worked with 30 investigators, 95% of who are CCC members affiliated with four CCC programs, and the amount of MASR service to these members increased 72% over the previous year. There are 42 current or pending grants from OSUCCC members that include utilization of the MASR. The OSUCCC has provided $2.1 M in Institutional support to establish and run the MASR as a "developing" Shared Resource. We now request CCSG funding that will provide `30% of the total support for this important full Shared Resource over the next five years.

在DNA或RNA水平上对多个基因的平行分析变得越来越重要，因为癌症被确定为涉及多种基因的疾病。为了研究这种一系列遗传改变，对单基因级别的分析不能成为最终答案。微阵列分析是最先进的技术满足这些科学需求。 OSUCCC微阵列共享资源（MASR）被确定为 1998年，“开发”共享资源最初为分子生物学和癌症遗传学的研究者提供服务具有基因组广泛表达分析的程序，随后成为共享资源之一整个OSUCCC使用Affymetrix Genechips提供基因组广泛的表达分析。 2002年9月，可用毛细管电泳对RNA完整性的定量，自2003年9月以来 MASR通过基于阵列的实时PCR的引物设计提供了微阵列结果的验证。为了使用这些技术中的任何一项，MASR提供多种服务，包括实验设计咨询，样本处理，杂交，扫描，完整的统计分析和咨询以及验证结果。较新的服务，例如自定义阵列和SNP，即将提供。 MASR火车研究人员将其结果上传到基因表达综合（http://www.ncbi.nlm.nlm.nih.gov/geo/）之后在许多期刊上都必须出版。 MASR网站（www.dnaarrays.org）包含有关服务，出版物和可下载协议和订单表格的信息。 MASR及时提供技术和专业专业知识，以竞争性的价格促进高质量的科学。支持 OSUCCC研究人员，每年处理几百个RNA样品以进行基因组范围的表达分析。此外，处理了几百个RNA样品以进行完整性定量。在2003年 MASR与30名调查员合作，其中95％是CCC成员隶属于四个CCC计划，这些成员的MASR服务数量比上一年增加了72％。有42个电流或在OSUCCC成员的赠款中，包括使用MASR。 OSUCCC提供了$ 2.1 m 在机构支持中，将MASR作为“开发”共享资源建立和运行。我们现在要求 CCSG资金将在接下来的五个中提供对这一重要共享资源的总支持的30％年。