Polymer Dot Nanoparticles for Detection of Single Molecules in Live Cells

用于检测活细胞中单分子的聚合物点纳米颗粒

基本信息

  • 批准号:
    7489459
  • 负责人:
  • 金额:
    $ 24.82万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-09-01 至 2011-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop a novel class of fluorescent nanoparticles called "Polymer Dots" and test the feasibility of the nanoparticles for detection of single molecules in live cells using conventional fluorescence microscopy methods. Polymer dot nanoparticles are a promising new fluorescent nanoparticle technology based on fluorescent pi-conjugated polymers which provide substantial improvements in brightness as compared to conventional fluorescent dyes and nanoparticles. The specific aims of the project include optimizing and characterizing relevant figures of merit for single molecule detection, development and testing of bioconjugation strategies for targeting specific biomolecules within cells, and demonstration of single nanoparticle detection in living cells. A range of characterization techniques will be employed, including fluorescence spectroscopy, single molecule spectroscopy, atomic force microscopy, electrophoresis, and epifluorescence microscopy. Improvements in brightness and photostability of a factor of 100 to 10,000 as compared to conventional fluorescent dyes are expected, meeting the requirements for fluorescence-based single molecule detection in living cells. Demonstration of facile, flexible bioconjugation methods for targeting specific biomolecules of interest and detection of individual labeled biomolecules within living cells are also expected. The development of this novel fluorescent nanoparticle platform will facilitate future research involving monitoring transport and biochemical events of single molecules within living cells and could also provide the basis for the development of novel bioassays and biosensors.
描述(申请人提供):拟议研究的目标是开发一种名为“聚合物点”的新型荧光纳米颗粒,并测试该纳米颗粒用于使用传统荧光显微镜方法检测活细胞中单分子的可行性。聚合物点纳米粒子是一种很有前途的新型荧光纳米粒子技术,它基于荧光聚酰亚胺共轭聚合物,与传统的荧光染料和纳米粒子相比,在亮度上有很大的提高。该项目的具体目标包括优化和表征单分子检测的相关优值系数,开发和测试针对细胞内特定生物分子的生物偶联策略,以及展示在活细胞中检测单个纳米颗粒。将使用一系列表征技术,包括荧光光谱、单分子光谱、原子力显微镜、电泳和荧光显微镜。与传统的荧光染料相比,在亮度和光稳定性方面有望提高100到10,000倍,满足活细胞中基于荧光的单分子检测的要求。还有望展示简便、灵活的生物偶联方法,用于靶向感兴趣的特定生物分子,并检测活细胞中个别标记的生物分子。这种新型荧光纳米颗粒平台的发展将促进未来涉及监测活细胞内单分子运输和生化事件的研究,并可能为新型生物检测和生物传感器的开发提供基础。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Jason Douglas McNeill其他文献

Jason Douglas McNeill的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Jason Douglas McNeill', 18)}}的其他基金

Polymer Dot Nanoparticles for Detection of Single Molecules in Live Cells
用于检测活细胞中单分子的聚合物点纳米颗粒
  • 批准号:
    7282916
  • 财政年份:
    2007
  • 资助金额:
    $ 24.82万
  • 项目类别:
Polymer Dot Nanoparticles for Detection of Single Molecules in Live Cells
用于检测活细胞中单分子的聚合物点纳米颗粒
  • 批准号:
    7923936
  • 财政年份:
    2007
  • 资助金额:
    $ 24.82万
  • 项目类别:
Polymer Dot Nanoparticles for Detection of Single Molecules in Live Cells
用于检测活细胞中单分子的聚合物点纳米颗粒
  • 批准号:
    7684801
  • 财政年份:
    2007
  • 资助金额:
    $ 24.82万
  • 项目类别:

相似海外基金

Pushing the envelope: atomic force microscopy imaging of the bacterial outer membrane during growth and division
挑战极限:生长和分裂过程中细菌外膜的原子力显微镜成像
  • 批准号:
    BB/X007669/1
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Research Grant
Nanoscopic elucidation of dynamic behavior of RNA viral nucleocapsid proteins using high-speed atomic force microscopy (HS-AFM)
使用高速原子力显微镜 (HS-AFM) 纳米级阐明 RNA 病毒核衣壳蛋白的动态行为
  • 批准号:
    24K18449
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Unravelling dengue virus structural dynamics and conformational changes using high-speed atomic force microscopy
使用高速原子力显微镜揭示登革热病毒结构动力学和构象变化
  • 批准号:
    24K18450
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Atomic scale reactivity of small islands of a bimetallic alloy on ceria to small molecules investigated by ultrahigh resolution atomic force microscopy
通过超高分辨率原子力显微镜研究二氧化铈上双金属合金小岛对小分子的原子尺度反应性
  • 批准号:
    24K01350
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
State-of-the-art atomic force microscopy facilities for South Australia
南澳大利亚最先进的原子力显微镜设施
  • 批准号:
    LE240100129
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Linkage Infrastructure, Equipment and Facilities
Pushing the envelope: atomic force microscopy imaging of the bacterial outer membrane during growth and division
挑战极限:生长和分裂过程中细菌外膜的原子力显微镜成像
  • 批准号:
    BB/X00760X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Research Grant
A New Nano Tip Fabrication Technique for Atomic Force Microscopy
原子力显微镜的新型纳米尖端制造技术
  • 批准号:
    DP230100637
  • 财政年份:
    2023
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Discovery Projects
Magnetic imaging by the locally induced anomalous Nernst effect using atomic force microscopy
使用原子力显微镜通过局部诱发的异常能斯特效应进行磁成像
  • 批准号:
    23K04579
  • 财政年份:
    2023
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Characterization of super adhesive aerosols on the basis of individual particle analysis using atomic force microscopy
基于原子力显微镜单个颗粒分析的超粘性气溶胶表征
  • 批准号:
    22KJ1464
  • 财政年份:
    2023
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Using atomic force microscopy to explore the processes and re-organisations that occur during bacterial growth and division and how these are influenc
使用原子力显微镜探索细菌生长和分裂过程中发生的过程和重组以及它们如何影响细菌
  • 批准号:
    2887441
  • 财政年份:
    2023
  • 资助金额:
    $ 24.82万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了