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Polymer Dot Nanoparticles for Detection of Single Molecules in Live Cells

用于检测活细胞中单分子的聚合物点纳米颗粒

基本信息

批准号：
7923936
负责人：
Jason Douglas McNeill
金额：
$ 24.52万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7923936
关键词：
Affinity Chromatography Atomic Force Microscopy Biochemical Biochemical Process Biological Assay Biosensor Blinking Carbohydrates Cell physiology Cells Chemistry Cytosol Detection Development Devices Dextrans Dimensions Disease Dyes Electrophoresis Encapsulated Endocytosis Event Extinction (Psychology)Fluorescence Fluorescence Microscopy Fluorescence Spectroscopy Fluorescent Dyes Fluorescent Probes Future Gene Expression Glass Image Individual Investigation Label Laboratories Laboratory Research Lasers Life Lighting Location Measures Membrane Methods Microscopy Monitor Organelles Peptides Phototoxicity Polymers Principal Investigator Process Property Protocols documentation Quantum Dots Research Research Personnel Semiconductors Signal Transduction Silicon Dioxide Solutions Spectrum Analysis Staining method Stains Structure Surface Suspension substance Suspensions Techniques Technology Testing Time Work absorption base cellular imaging cellular pathology cytotoxicity design dextran flexibility innovation interest meetings nanoparticle nanoscale novel particle programs protein transport quantum sensor single molecule solid state targeted delivery uptake

项目摘要

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop a novel class of fluorescent nanoparticles called "Polymer Dots" and test the feasibility of the nanoparticles for detection of single molecules in live cells using conventional fluorescence microscopy methods. Polymer dot nanoparticles are a promising new fluorescent nanoparticle technology based on fluorescent pi-conjugated polymers which provide substantial improvements in brightness as compared to conventional fluorescent dyes and nanoparticles. The specific aims of the project include optimizing and characterizing relevant figures of merit for single molecule detection, development and testing of bioconjugation strategies for targeting specific biomolecules within cells, and demonstration of single nanoparticle detection in living cells. A range of characterization techniques will be employed, including fluorescence spectroscopy, single molecule spectroscopy, atomic force microscopy, electrophoresis, and epifluorescence microscopy. Improvements in brightness and photostability of a factor of 100 to 10,000 as compared to conventional fluorescent dyes are expected, meeting the requirements for fluorescence-based single molecule detection in living cells. Demonstration of facile, flexible bioconjugation methods for targeting specific biomolecules of interest and detection of individual labeled biomolecules within living cells are also expected. The development of this novel fluorescent nanoparticle platform will facilitate future research involving monitoring transport and biochemical events of single molecules within living cells and could also provide the basis for the development of novel bioassays and biosensors.

描述（由申请人提供）：拟议研究的目的是开发一种称为“聚合物点”的新型荧光纳米颗粒，并测试纳米颗粒使用传统荧光显微镜方法检测活细胞中单分子的可行性。聚合物点纳米粒子是一种基于荧光偶联聚合物的新型荧光纳米粒子技术，与传统的荧光染料和纳米粒子相比，它的亮度有了很大的提高。该项目的具体目标包括优化和表征单分子检测的相关优点，开发和测试针对细胞内特定生物分子的生物偶联策略，以及在活细胞中进行单纳米颗粒检测的演示。一系列的表征技术将被采用，包括荧光光谱，单分子光谱，原子力显微镜，电泳和荧光显微镜。与传统荧光染料相比，亮度和光稳定性有望提高100到10,000倍，满足活细胞中基于荧光的单分子检测的要求。此外，还有望展示针对感兴趣的特定生物分子和检测活细胞内单个标记生物分子的简便、灵活的生物偶联方法。这种新型荧光纳米粒子平台的开发将促进未来研究涉及监测活细胞内单分子的运输和生化事件，也可以为新型生物测定和生物传感器的发展提供基础。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Multicolor conjugated polymer dots for biological fluorescence imaging.

DOI：
10.1021/nn800590n
发表时间：
2008-11-25
期刊：
ACS NANO
影响因子：
17.1
作者：
Wu, Changfeng;Bull, Barbara;Szymanski, Craig;Christensen, Kenneth;McNeill, Jason
通讯作者：
McNeill, Jason

Incorporating functionalized polyethylene glycol lipids into reprecipitated conjugated polymer nanoparticles for bioconjugation and targeted labeling of cells.

DOI：
10.1039/c0nr00746c
发表时间：
2011-03
期刊：
Nanoscale
影响因子：
6.7
作者：
Kandel PK;Fernando LP;Ackroyd PC;Christensen KA
通讯作者：
Christensen KA

Core-shell silver nanoparticles for optical labeling of cells.

用于细胞光学标记的核壳银纳米颗粒。

DOI：
10.1016/j.ab.2014.04.015
发表时间：
2014
期刊：
Analytical biochemistry
影响因子：
2.9
作者：
Dukes,KyleD;Christensen,KennethA;Chumanov,George
通讯作者：
Chumanov,George

Mechanism of cellular uptake of highly fluorescent conjugated polymer nanoparticles.

DOI：
10.1021/bm1007103
发表时间：
2010-10-11
期刊：
BIOMACROMOLECULES
影响因子：
6.2
作者：
Fernando, Lawrence P.;Kandel, Prakash K.;Yu, Jiangbo;McNeill, Jason;Ackroyd, P. Christine;Christensen, Kenneth A.
通讯作者：
Christensen, Kenneth A.

Ratiometric single-nanoparticle oxygen sensors for biological imaging.