Small Animal Imaging

基于分子的成像技术为评估体内重要的细胞过程提供了新的机会。有能力 通过非侵入性成像监测癌症的分子过程可能提供关键信息 关于治疗的效果。在临床前研究的背景下，体内成像的使用允许 获取每种动物的完整动态生物分布研究，从而减少 达到统计上适当结果所需的动物；通常用于小动物成像的技术 可直接移植到临床环境中。小动物影像核心(SAIC)是一种新的共享 致力于为研究人员提供访问最先进的小动物成像技术的资源 用于与希望癌症之城使命相关的基础和翻译研究的能力 中心。国家工商行政管理总局的具体目标包括：(1)深入了解当前 小动物成像在癌症研究中的能力和局限性；(2)实施， 开发、校准、维护和操作相关成像系统 动物成像实验室；以及(3)在希望之城的研究中优化小动物成像的使用 咨询调查人员。核心人员目前包括一名主任、一名成像物理学家和一名 他们都在使用成像技术进行动物研究方面经验丰富。小动物 运行中的成像系统包括两个用于生物发光光学成像的单元(I VIS 100，Xenogen 公司)；伽马相机(Y Imager，BiSpace，Inc.)；PET扫描仪(MicroPET R4，CTIMI，Inc.)；以及CT 扫描仪(microCAT II高分辨率，CTIMI，Inc.)。MicroPET和microCAT很容易串联使用 生成联合配准的功能解剖PET/CT图像。动物资源中心提供了 帕尔文生物医学研究大楼内供动物成像计划使用的四个房间(一个房间 用于microPET、microCAT和y-Imager；两个房间用于Xenogen MS机器；一个房间 用于伽马计数器)。已经开发了一种系统，用于向用户收取成像的部分费用 程序。在第一个12个月的报告期内，13个癌症中心成员使用了SAIC 来自3个研究项目和同行评审的使用量占总使用量的89%。年度预算： 核心是165,922美元(97%的机构，3%的按存储容量使用计费)；42%(70,000美元)正在向CCSG申请。

Molecular-based imaging provides new opportunities to assess vital cellular processes in vivo. The ability to monitor the molecular processes of cancer via non-invasive imaging may provide critical information regarding the effects of therapy. In the context of pre-clinical research, the use of in vivo imaging permits the acquisition of a complete dynamic biodistribution study in each animal, thereby reducing the number of animals required to reach a statistically adequate result; often the techniques used in small animal imaging are directly transferable to the clinical setting. The Small Animal Imaging Core (SAIC) is a new shared resource dedicated to providing investigators access to the state-of-the-art in small animal imaging capabilities for use in basic and translational research relevant to the mission of the City of Hope Cancer Center. Specific aims of the SAIC include: (1) maintaining a thorough understanding of the current capabilities and limitations of small animal imaging as they pertain to cancer research; (2) implementing, developing, calibrating, maintaining, and operating relevant imaging systems within the context of a small animal imaging laboratory; and (3) optimizing the use of small animal imaging in research at City of Hope by consulting with investigators. Core personnel currently include a Director, an imaging physicist, and a manager, all of whom are highly experienced in the use of imaging for research with animals. Small animal imaging systems in operation include two units for bioluminescence optical imaging (I VIS 100, Xenogen Corp.); a gamma camera (Y IMAGER, Biospace, Inc.); a PET scanner (microPET R4, CTIMI, Inc.); and a CT scanner (microCAT II Hi Res, CTIMI, Inc.). The microPET and microCAT are readily used in tandem to generate co-registered functional-anatomic PET/CT images. The Animal Resources Center has provided four rooms within the Parvin Biomedical Research Building for use by the animal imaging program (one room for the microPET, microCAT, and the y-IMAGER; two rooms for the Xenogen MS machines; and one room for a gamma counter). A system has been developed for billing users for a portion of the costs of the imaging procedures. During the first 12-month reporting period, the SAIC was used by 13 Cancer Center members from 3 Research Programs and peer-reviewed usage represented 89% of total usage. Annual budget for the core is $165,922 (97% institution, 3% chargebacks); 42% ($70,000) is being requested from the CCSG.