Analytical Cytochemistry

分析细胞化学

• 批准号: 8518642
• 负责人: THEODORE G KRONTIRIS
• 金额: $ 14万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2013-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8518642
• 关键词: Archives; Back; Biological Assay; Budgets; Calibration; Cancer Center; Cancer Center Support Grant; Cells; Computer software; Cytometry; Data; Development; Equipment; Fees; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Goals; Image; Label; Laboratories; Laser Scanning Cytometry; Lasers; Libraries; Logistics; Maintenance; Management Information Systems; Measures; Microscopic; Network-based; Peer Review; Population; Preparation; Preventive; Process; Property; Publications; Quality Control; Research Personnel; Resources; Retrieval; Running; Scanning; Screening procedure; Services; Sorting - Cell Movement; Speed; Technology; Time; Tissues; Training; base; computerized data processing; cost; cytochemistry; experience; instrumentation; light scattering; member; operation; programs; research study; tool

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.

分析细胞术核心（ACC）的目标是提供先进的设备和经验丰富的操作员来测量和分离细胞及其成分的特性，并提供内部分析和外部审查的数据。该核心资源中的流式细胞仪包括(1)高速细胞分选仪（MoFlo）和(2)分析细胞仪（Cyan和FacsCalibur）。流式细胞仪仪器为研究人员提供了工具，以高达35,000个细胞/秒的速度分析和分离细胞，基于多个荧光标记和高产量的光散射特性（基于速度高达90%）