Analytical Cytochemistry

分析细胞化学

• 批准号: 7714116
• 负责人: THEODORE G KRONTIRIS
• 金额: $ 4.97万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714116
• 关键词: Archives; Budgets; Calibration; Cancer Center; Cancer Center Support Grant; Cells; Computer software; Cytometry; Data; Equipment; Fees; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Goals; Image; Knowledge; Label; Laboratories; Laboratory Research; Lasers; Libraries; Logistics; Maintenance; Management Information Systems; Measures; Microscopic; Network-based; Peer Review; Population; Preparation; Preventive; Property; Publications; Quality Control; Research Personnel; Resource Sharing; Resources; Retrieval; Running; Sampling; Scanning; Screening procedure; Sorting - Cell Movement; Speed; Technology; Time; Tissues; Training; Update; base; computerized data processing; cost; cytochemistry; experience; human tissue; instrumentation; light scattering; member; programs; research study; tool

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.

分析细胞计数核心（ACC）的目标是提供领先的设备和经验丰富的 操作员测量细胞及其成分的特性并分离细胞及其成分，并提供内部数据 分析和外部审查。流式细胞仪仪器在这一核心资源包括（1）高速 细胞分选仪（MoFlo）和（2）分析细胞仪（Cyan和FacsCalibur）。流式细胞仪 为研究人员提供了分析和分离细胞的工具，速度高达35，000个细胞/秒 基于多种荧光标记和光散射特性，产量高（基于速度高达90%） 纯度极高（99%）。由于能够在多个数据库上同时对四个种群进行排序， 调查人员的工作参数、后勤和费用都大大减少。新的高吞吐量 Cyan分析仪的平板取样器为研究者提供了快速筛选大型化合物的能力 在化合物鉴定中使用流式细胞仪分析的图书馆;增加激光扫描 细胞仪（LSC）已扩大了ACC提供的分析能力，包括定量组织 荧光显微镜该仪器收集列表模式荧光数据， 显微图像，并为用户提供将定量荧光数据与 大组织切片上的定性显微图像。LSC由经过专门培训的用户运行， 由专门的运营商管理。ACC仪器每周接受质量控制评估， 例行预防性维护和校准。核心中生成的数据可通过BRI-net获得 用于进一步分析和准备演示或出版的服务器。基于网络的数据处理 软件由核心提供，实验室信息管理系统（LIMS）正在开发中 以帮助跟踪实验相关的Meta数据和存档文件检索。在2006财政年度， 来自所有5个项目的44名癌症中心成员和3名非结盟成员（64%的同行评审使用率）。 这一核心资源的年度预算为401，799美元（43%为机构经费，40%为用户费用）; 17%（70，100美元）来自 CCSG。