Analytical Cytochemistry

分析细胞化学

• 批准号: 7714116
• 负责人: THEODORE G KRONTIRIS
• 金额: $ 4.97万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714116
• 关键词: Archives; Budgets; Calibration; Cancer Center; Cancer Center Support Grant; Cells; Computer software; Cytometry; Data; Equipment; Fees; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Goals; Image; Knowledge; Label; Laboratories; Laboratory Research; Lasers; Libraries; Logistics; Maintenance; Management Information Systems; Measures; Microscopic; Network-based; Peer Review; Population; Preparation; Preventive; Property; Publications; Quality Control; Research Personnel; Resource Sharing; Resources; Retrieval; Running; Sampling; Scanning; Screening procedure; Sorting - Cell Movement; Speed; Technology; Time; Tissues; Training; Update; base; computerized data processing; cost; cytochemistry; experience; human tissue; instrumentation; light scattering; member; programs; research study; tool

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.

分析细胞学核心(ACC)的目标是提供领先的设备和经验丰富的 运算符用于测量和隔离细胞及其组件的属性，并将数据提供给内部 分析和外部审查。这一核心资源中的流式细胞仪包括(1)高速 细胞分选仪(MoFlo)和(2)分析细胞仪(Cyan和FacsCalibur)。流式细胞仪 为调查人员提供分析和分离细胞的工具，速度最高可达35,000个细胞/秒 基于多个荧光标签和光散射特性，产量高(基于速度高达90%) 和极高的纯度(99%)。由于能够一次对四个种群在多个 参数，大大减少了调查人员的后勤和成本。一种新的高吞吐量 青色分析器的平板取样器为研究人员提供了快速筛选大化合物的能力 在化合物鉴定中使用流式细胞术分析的图书馆；增加激光扫描 细胞仪(LSC)扩展了ACC提供的分析能力，以包括基于定量组织的 荧光显微镜。此工具收集列表模式荧光数据和 显微图像，并为用户提供将定量荧光数据与 大组织切片上的定性显微图像。LSC由经过专门培训的用户运行， 由专门的运营商管理。ACC仪器设备每周接受质量控制评估，并 日常预防性维护和校准。在核心中产生的数据可通过一带一路网络获得 服务器，用于进一步分析和准备演示文稿或出版物。基于网络的数据处理 核心提供软件，实验室信息管理系统(LIMS)正在开发中 以帮助跟踪与实验相关的元数据和归档文件检索。在2006财年，使用了ACC 来自所有5个项目的44名癌症中心成员和3名非对齐成员(%同行评议使用率)。 这一核心的年度预算为401,799美元(43%的机构费用，40%的用户费用)；17%(70,100美元)的申请来自 CCSG。