Molecular Cytogenetics

分子细胞遗传学设施（MCF）为基础和临床研究人员提供常规和 基于FISH的细胞遗传学服务。核心的目标是提供1）高品质，合理的价格 为请求研究者提供细胞遗传学服务和专业知识; 2）标准染色体核型分析和G， C、Q、R、银染色，以确定染色体异常，作为基因组不稳定性的证据; 3)荧光原位杂交（FISH）的中期扩散，使用市售 染色体涂料探针、基因座特异性探针和端粒探针; 4）光谱核型分析（SKY） 用于在一个杂交反应中明确鉴定小鼠或人染色体 易位存在于中期扩散; 5）专门的服务，如端粒长度 根据需要，通过定量FISH（Q-FISH）进行测定;以及6）接触 在各种细胞遗传学技术方面经验丰富， 自己MCF避免了个别教师实验室的人员，设施， 以及使用这些强大工具所需的设备。该设施占地250平方米。英尺内 癌症遗传学系。新增200平方米英尺显微镜室容纳4个荧光 用于染色体分析的显微镜。MCF配备有一名主任、一名联合主任、一个实验室 助理和名誉教授在上一个资助期内，共收集了741个样本， 常规和基于FISH的细胞遗传学服务。同行资助的研究人员优先考虑 来自19个CCSG项目中的18个项目的76名研究者使用了MCF服务。92%的用户进行了同行评审 资金，占使用率的85%。自成立以来，MCF成功地 支持5个同行评审的NIH资助的赠款和研究人员使用MCF产生的数据， 在顶级期刊上广泛发表未来的计划是建立新的细胞遗传学服务， 升级现有技术。

The Molecular Cytogenetics Facility (MCF) provides basic and clinical investigators with conventional and FISH-based cytogenetic services. The goals of the core are to provide 1) high-quality, reasonably-priced cytogenetic services and expertise for requesting investigators; 2) standard chromosome karyotyping and G, C, Q, R, silver staining to ascertain chromosomal abnormalities for evidence of genomic instability; 3) fluorescence in situ hybridization (FISH) of metaphase spreads using commercially- available chromosome paint probes, locus-specific probes, and telomere probes; 4) Spectral Karyotyping (SKY) service to unambiguously identify, in one hybridization reaction, mouse or human chromosomal translocations present within a metaphase spread; 5) specialized services, such as telomere length determination by Quantitative-FISH (Q-FISH), on an as-needed basis; and 6) access to personnel who are highly-experienced in a variety of cytogenetic technologies as well as access to the methodologies themselves. The MCF avoids expensive duplication in individual faculty laboratories of personnel, facilities, and equipment required for application of these powerful tools. The Facility occupies 250 sq. ft. within the Department of Cancer Genetics. An additional 200 sq. ft. microscope room houses 4 fluorescent microscopes for chromosomal analyses. The MCF is staffed with a Director, a Co-Director, 1 Laboratory Assistant, 'and a Professor Emeritus. During the previous funding period, 741 specimens were collected for both conventional and FISH-based cytogenetic services. Peer-funded investigators are given priority for MCF services that were used by 76 investigators from 18 of the 19 CCSG programs. 92% of users had peerreviewed funding and accounted for 85% of utilization. Since its inception, the MCF has successfully supported 5 peer-reviewed NIH-funded grants and investigators using data generated by the MCF have published extensively in top-tier journals. Future plans are to establish new cytogenetic services as well as to upgrade existing technologies.