Genetically Engineered Mouse Facility

转基因小鼠已成为癌症动物模型的“黄金标准”。它们广泛存在于 用于模拟具有肿瘤抑制基因特定点突变的人类癌症，以及组织特异性 突变在体细胞组织中的表达。通过在一种动物身上结合几种突变，现在是 有可能复制以前难以在动物模型中复制的人类癌症。这个 基因工程老鼠设施(GEMF)为癌症中心成员提供了宝贵的资源。它 产生转基因和基因靶向的小鼠，进行胚胎再衍生以产生无病原体 小鼠，并提供冷冻保存服务以存档动物模型。鼠标资源工具 (MRF)，作为GEMF的一部分，为构建发育、成虫和 胚胎组织、基因组DNA和cdna文库以及袋式过滤器。MRF也有一个很小的殖民地 Cre和LacZ转基因小鼠对小鼠产生条件性缺失至关重要。因此，GEMF 为教职员工提供独特的机会来开发复杂的动物模型来研究各种癌症 有问题。GEMF在生成和维护老鼠模型方面非常成功。在 在过去的五年里，GEMF为MDACC调查人员制作了300多个项目的动物模型 并已冷冻保存了120多个小鼠品系。A&GT；实现了1000%的利用率 鼠标资源通过MRF。首席设施协调员开发了新的、更有效的方法 产生供该设施使用的胚胎。她负责培训教职员工，以及 为许多不同项目的研究人员提供遗传和技术方面的专业知识。除 作为设施协调员，GEMF由7名训练有素的技术人员组成。在过去的五年中，GEMF 已经为96个不同的用户提供了服务，他们代表了20个赞助节目中的19个；97%的调查人员 提供服务的是MDACC教职员工。为了更好地满足我们调查人员的需求并增加所需的能力， 南校区最近增加了卫星设施。这一扩展将使GEMF能够发展 更多型号，并提供额外的服务。全球环境管理基金目前的资金来源有多种，包括 53%由CCSG提供，45%通过用户费用收回，其余部分由MDACC提供。

Genetically engineered mice have become the "gold standard" for animal models in cancer. They are widely used to mimic human cancers with specific point mutations in tumor suppressor genes, as well as tissuespecific expression of mutations in somatic tissues. By combining several mutations in one animal, it is now possible to phenocopy human cancers that were difficult to reproduce previously in animal models. The Genetically Engineered Mouse Facility (GEMF) provides valuable resources to Cancer Center members. It generates transgenic and gene-targeted mice, performs embryo rederivations to generate pathogen-free mice, and provides cryopreservation services to archive animal models. The Mouse Resource Facility (MRF), as part of the GEMF, provides vectors for construct development, northern blots of adult and embryonic tissues, genomic DNA and cDNA libraries, and BAG filter sets. The MRF also has a small colony of Cre and lacZ transgenic mice essential for generating conditional deletions in mice. The GEMF thus provides unique opportunities to faculty to develop sophisticated animal models to study a variety of cancer problems. The GEMF has been extremely successful in generating and maintaining mouse models. In the last five years, the GEMF has generated animal models for more than 300 projects for MDACC investigators and has cryopreserved more than 120 mouse lines. A >1000% increase has been achieved in utilization of mouse resources through the MRF. The lead facility coordinator has developed new, more efficient methods of generating embryos for use by the facility. She is responsible for training faculty and their staff, and provides genetic and technical expertise to many investigators in many different programs. In addition to the facility coordinator, the GEMF is staffed by 7 highly-trained technicians. In the past five years, the GEMF has served 96 different users who represent 19 of the 20 sponsored programs; 97% of the investigators served are MDACC faculty. To better serve the needs of our investigators and add needed capacity, a small satellite facility was added recently to the South Campus. This expansion will enable the GEMF to develop more models and offer additional services. The GEMF is currently funded from multiple sources, including 53% provided by the CCSG and 45% recovered through user fees, with the remainder provided by MDACC.