Genetically Engineered Mouse Facility

基因工程小鼠设施

• 批准号: 7695935
• 负责人: WILLIAM H. KLEIN
• 金额: $ 34.35万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-28 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7695935
• 关键词: Adult; Advertising; Animal Model; Animals; Archives; Bacterial Artificial Chromosomes; Cancer Center; Cancer Center Support Grant; Complex; Cryopreservation; DNA; Derivation procedure; Detection; Development; Embryo; Faculty; Fees; Funding; Gene Targeting; Generations; Genes; Genetic; Genetic Engineering; Genetic Variation; Genetically Engineered Mouse; Genomics; Gold; Green Fluorescent Proteins; Growth; Human; Immunology; Injection of therapeutic agent; LacZ Genes; Lead; Malignant Neoplasms; Methods; Mission; Modeling; Mus; Mutation; Northern Blotting; Numbers; Phenocopy; Phenotype; Pliability; Point Mutation; Procedures; Processed Genes; Range; Reagent; Reproductive Immunology; Research Personnel; Resources; Services; Source; Standards of Weights and Measures; TP53 gene; Technical Expertise; Techniques; Testing; Time; Tissues; Training; Transgenic Mice; Transgenic Organisms; Tumor Suppressor Genes; University of Texas M D Anderson Cancer Center; blastocyst; cDNA Library; embryo tissue; embryonic stem cell; germ free condition; in vivo; member; mouse genome; mouse model; pathogen; plasmid DNA; programs; tumor; vector

Genetically engineered mice have become the "gold standard" for animal models in cancer. They are widely used to mimic human cancers with specific point mutations in tumor suppressor genes, as well as tissuespecific expression of mutations in somatic tissues. By combining several mutations in one animal, it is now possible to phenocopy human cancers that were difficult to reproduce previously in animal models. The Genetically Engineered Mouse Facility (GEMF) provides valuable resources to Cancer Center members. It generates transgenic and gene-targeted mice, performs embryo rederivations to generate pathogen-free mice, and provides cryopreservation services to archive animal models. The Mouse Resource Facility (MRF), as part of the GEMF, provides vectors for construct development, northern blots of adult and embryonic tissues, genomic DNA and cDNA libraries, and BAG filter sets. The MRF also has a small colony of Cre and lacZ transgenic mice essential for generating conditional deletions in mice. The GEMF thus provides unique opportunities to faculty to develop sophisticated animal models to study a variety of cancer problems. The GEMF has been extremely successful in generating and maintaining mouse models. In the last five years, the GEMF has generated animal models for more than 300 projects for MDACC investigators and has cryopreserved more than 120 mouse lines. A >1000% increase has been achieved in utilization of mouse resources through the MRF. The lead facility coordinator has developed new, more efficient methods of generating embryos for use by the facility. She is responsible for training faculty and their staff, and provides genetic and technical expertise to many investigators in many different programs. In addition to the facility coordinator, the GEMF is staffed by 7 highly-trained technicians. In the past five years, the GEMF has served 96 different users who represent 19 of the 20 sponsored programs; 97% of the investigators served are MDACC faculty. To better serve the needs of our investigators and add needed capacity, a small satellite facility was added recently to the South Campus. This expansion will enable the GEMF to develop more models and offer additional services. The GEMF is currently funded from multiple sources, including 53% provided by the CCSG and 45% recovered through user fees, with the remainder provided by MDACC.

基因工程小鼠已成为癌症动物模型的“黄金标准”。它们被广泛 用于模拟肿瘤抑制基因中具有特定点突变的人类癌症，以及组织特异性 体细胞组织中突变的表达。通过在一种动物中结合几种突变， 这可能使人类癌症的表型复制成为可能，而这些癌症以前很难在动物模型中复制。的 基因工程小鼠设施（GEMF）为癌症中心成员提供了宝贵的资源。它 产生转基因和基因靶向小鼠，进行胚胎再衍生以产生无病原体的 小鼠，并提供冷冻保存服务，以存档动物模型。老鼠资源设施 (MRF)作为GEMF的一部分，提供了构建体发育的载体，成虫和成虫的北方印迹， 胚胎组织、基因组DNA和cDNA文库以及BAG过滤器组。MRF也有一个小殖民地 的Cre和lacZ转基因小鼠在小鼠中产生条件性缺失所必需的。因此，GEMF 提供了独特的机会，教师开发复杂的动物模型，以研究各种癌症 问题GEMF在建立和维持小鼠模型方面非常成功。在 在过去的五年里，GEMF为MDACC的研究人员提供了300多个项目的动物模型 已冻存小鼠品系120余只。利用率提高了1000%以上， 鼠标资源通过MRF.首席设施协调员开发了新的更有效的方法 生产供实验室使用的胚胎她负责培训教职员工， 为许多不同项目的研究人员提供遗传学和技术专业知识。除了有 GEMF由7名训练有素的技术人员组成。在过去的五年里，GEMF 已经为96个不同的用户提供了服务，他们代表了20个赞助项目中的19个; 97%的调查人员 服务是MDACC教师。为了更好地满足我们调查人员的需求并增加所需的容量， 南校区最近增加了卫星设施。这一扩张将使GEMF能够发展 更多的模式和提供额外的服务。GEMF目前由多个来源供资，包括 53%由CCSG提供，45%通过用户费收回，其余由MDACC提供。