CORE 1 TRP3: PRODUCT TERMINAL ISOTOPE CODING (PROTIC)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 技术研究项目3，产品终端同位素编码（PROTIC），将重点放在 蛋白质水解终产物的蛋白质组学表征。识别所有产品的能力，以及 然后推断初始底物，因为蛋白酶本质上是蛋白水解的“圣杯”。这 这种能力将改变细胞生物学领域。 这项任务的重要性与它的难度相称。我们建议开发一种全新的化学策略，用于产物末端同位素编码（PROTIC），即生物样品中所有N-末端的同位素标记。这种标记方法将允许识别和相对 复杂蛋白质组中底物/产物谱的定量

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technical Research Project 3, Product Terminal Isotope Coding (PROTIC), will focus on the proteomic characterization of the end products of proteolysis. The ability to identify all products, and then infer the initial substrates, for a protease is essentially the "holy grail" of proteolysis. This capability would transform the field of cell biology. The significance of the task is matched by its difficulty. We propose to develop an entirely novel chemical strategy for Product Terminal Isotope Coding (PROTIC), the isotope tagging of all N-termini in biological samples. This tagging method will allow the identification and relative quantification of substrate/product profiles in complex proteomes