IMPAIRED ADIPOGENESIS IN INSULIN RESISTANCE

胰岛素抵抗中脂肪生成受损

• 批准号: 7952122
• 负责人: RICHARD E PRATLEY
• 金额: $ 2.42万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7952122
• 关键词: Address; Adipocytes; Adult; Age; Biopsy; Body fat; Buttocks; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Diagnosis; Dual-Energy X-Ray Absorptiometry; Fatty acid glycerol esters; Funding; Gender; Grant; Growth; Hormones; Individual; Infusion procedures; Institution; Insulin; Insulin Resistance; Label; Laboratories; Lead; Measurement; Measures; Metabolism; Needles; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Patients; Radioactive; Recruitment Activity; Research; Research Personnel; Resources; Risk; Risk Factors; Sampling; Scanning; Series; Skin; Source; Testing; United States National Institutes of Health; Water; drinking; insulin sensitivity; lipid biosynthesis; new growth; oral glucose tolerance; prevent; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Obesity is a strong risk factor for developing type 2 diabetes (T2DM), but the reasons for this are not fully understood. In particular, it is not known why some obese people develop T2DM while other obese individuals do not. This study tests whether differences in fat cells (adipocytes) are to blame. Even in adults, fat cells are constantly being formed to replace old fat cells and to respond to the body's need to store excess energy. The ability to form new fat cells may be diminished in some individuals, leading to larger fat cells. These large fat cells secrete hormones that may increase risk for T2DM. This study tests whether fat cells from obese insulin resistant subjects (who are at risk for developing T2DM) form at a slower rate than those from insulin sensitive subjects (who are at lower risk for developing T2DM). To address this question we will recruit and study two groups of obese subjects, selected to be similar in age, gender and degree of obesity. One group of subjects will be obese and insulin resistant (the OIR group), while the other will be comparably obese, but insulin sensitive (OIS). Subjects will undergo a series of studies to characterize their metabolism including measurement of body fat by DXA scanning, oral glucose tolerance (a test used to diagnose diabetes) and measurement of insulin sensitivity in response to an infusion of insulin (a research study used to classify patients into the OIR and OIS groups). Small samples of fat (from just under the skin of the belly and the buttocks) will obtained using a needle on two occasions over 12 weeks. During these 12 weeks, subjects will drink a small amount of water that contains a non-radioactive label. This labeled water will allow us to measure the rate of growth of new fat cells in the body. We will also look at the rate of growth of fat cells obtained from these biopsies in the laboratory. The results of this study may tell us more about why certain obese people develop diabetes and why others do not. This might lead to new ways to prevent or treat T2DM.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 肥胖是发展2型糖尿病（T2 DM）的一个强有力的危险因素，但其原因尚不完全清楚。特别是，目前尚不清楚为什么一些肥胖的人发展T2 DM，而其他肥胖的人没有。这项研究测试了脂肪细胞（脂肪细胞）的差异是否是罪魁祸首。即使在成年人中，脂肪细胞也在不断形成，以取代旧的脂肪细胞，并响应身体储存多余能量的需要。在某些个体中，形成新脂肪细胞的能力可能会减弱，导致更大的脂肪细胞。这些大的脂肪细胞分泌的激素可能会增加T2 DM的风险。本研究测试了肥胖胰岛素抵抗受试者（有发生T2 DM的风险）的脂肪细胞形成速度是否慢于胰岛素敏感受试者（发生T2 DM的风险较低）。 为了解决这个问题，我们将招募和研究两组肥胖受试者，选择在年龄，性别和肥胖程度相似。一组受试者肥胖且胰岛素抵抗（OIR组），而另一组受试者相对肥胖，但胰岛素敏感（OIS）。受试者将接受一系列研究以表征其代谢，包括通过DXA扫描测量体脂、口服葡萄糖耐量（用于诊断糖尿病的测试）和测量胰岛素输注后的胰岛素敏感性（用于将患者分为OIR和OIS组的研究）。在12周内，将使用针头在两个场合获得少量脂肪样本（来自腹部和臀部皮肤下）。在这12周内，受试者将饮用少量含有非放射性标记的水。这种标记的水将使我们能够测量体内新脂肪细胞的生长速度。我们还将在实验室中观察从这些活检中获得的脂肪细胞的生长速度。 这项研究的结果可能会告诉我们更多关于为什么某些肥胖的人患糖尿病，为什么其他人没有。 这可能会导致预防或治疗T2 DM的新方法。