IMPAIRED ADIPOGENESIS IN INSULIN RESISTANCE: PILOT CLINICAL AMP IN VITRO STUDIES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肥胖是罹患2型糖尿病(T2 DM)的强烈风险因素，但其原因尚不完全清楚。特别是，目前还不知道为什么一些肥胖者会患上T2 DM，而其他肥胖者则不会。这项研究测试了脂肪细胞(脂肪细胞)的差异是否是罪魁祸首。即使在成年人中，脂肪细胞也在不断地形成，以取代陈旧的脂肪细胞，并对身体储存多余能量的需求做出反应。在某些个体中，形成新脂肪细胞的能力可能会减弱，导致脂肪细胞变大。这些巨大的脂肪细胞分泌的激素可能会增加患T2 DM的风险。这项研究测试了肥胖的胰岛素抵抗受试者(他们有患T2 DM的风险)的脂肪细胞形成速度是否比那些对胰岛素敏感的受试者(患T2 DM的风险更低)的脂肪细胞形成速度慢。 为了解决这个问题，我们将招募和研究两组肥胖受试者，他们被选为年龄、性别和肥胖程度相似的人。一组受试者将患有肥胖和胰岛素抵抗(OIR组)，而另一组受试者将相对肥胖，但对胰岛素敏感(OIS)。受试者将接受一系列研究，以表征他们的新陈代谢，包括通过DXA扫描测量身体脂肪，口服葡萄糖耐量(一种用于诊断糖尿病的测试)，以及测量对注射胰岛素的胰岛素敏感性(一项用于将患者分为OIR和OIS组的研究研究)。在12周内，将使用针头两次采集小块脂肪样本(取自腹部和臀部皮肤下)。在这12周内，受试者将饮用少量含有非放射性标签的水。这种有标签的水将使我们能够测量体内新脂肪细胞的生长速度。我们还将观察从实验室的活组织检查中获得的脂肪细胞的生长速度。 这项研究的结果可能会告诉我们更多关于为什么某些肥胖者会患糖尿病，为什么其他人不会。这可能会带来预防或治疗T2 DM的新方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Obesity is a strong risk factor for developing type 2 diabetes (T2DM), but the reasons for this are not fully understood. In particular, it is not known why some obese people develop T2DM while other obese individuals do not. This study tests whether differences in fat cells (adipocytes) are to blame. Even in adults, fat cells are constantly being formed to replace old fat cells and to respond to the body's need to store excess energy. The ability to form new fat cells may be diminished in some individuals, leading to larger fat cells. These large fat cells secrete hormones that may increase risk for T2DM. This study tests whether fat cells from obese insulin resistant subjects (who are at risk for developing T2DM) form at a slower rate than those from insulin sensitive subjects (who are at lower risk for developing T2DM). To address this question we will recruit and study two groups of obese subjects, selected to be similar in age, gender and degree of obesity. One group of subjects will be obese and insulin resistant (the OIR group), while the other will be comparably obese, but insulin sensitive (OIS). Subjects will undergo a series of studies to characterize their metabolism including measurement of body fat by DXA scanning, oral glucose tolerance (a test used to diagnose diabetes) and measurement of insulin sensitivity in response to an infusion of insulin (a research study used to classify patients into the OIR and OIS groups). Small samples of fat (from just under the skin of the belly and the buttocks) will obtained using a needle on two occasions over 12 weeks. During these 12 weeks, subjects will drink a small amount of water that contains a non-radioactive label. This labeled water will allow us to measure the rate of growth of new fat cells in the body. We will also look at the rate of growth of fat cells obtained from these biopsies in the laboratory. The results of this study may tell us more about why certain obese people develop diabetes and why others do not. This might lead to new ways to prevent or treat T2DM.