DNA MICROARRAY AND EXPRESSION CORE

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression Core (Microarray Core) at TNPRC has functioned for the last five years, with a mandate to provide Microarray and Protein expression services to the researchers here. Over the last two years, the DNA Core has enhanced its infrastructure and capabilities, and currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design: 2) Spotted or 2-color microarray experiments: 3) Microarray Data Analysis 4) Small Sample Amplification 5) PCR Arrays 6) RNA Isolation B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community 2) Optimization and standardization of individual siRNA assay C. Protein Expression and Purification: 1) Expression and purification of proteins of interest in E. coli. In the coming years, the core would like to expand into the following research service areas: 1. Custom microarrays: The core will produce several custom microarrays based on demand. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony. The core is working closely with the Viral Diagnostics core to generate the multi-pathogen array. 2. Expression and Purification of Modified Proteins: In order to express and purify proteins with eukaryotic modifications such as glycosylation, the core will expand to offer expression service in hosts other than E.coli e.g. Drosophila.