MODIFICATION OF A COMMON BAL TECHNIQUE TO ENHANCE SAMPLE DIAGNOSTIC VALUE

改进通用平衡技术以提高样品诊断价值

• 批准号: 7716330
• 负责人: Morgan Singletary
• 金额: $ 2.41万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716330
• 关键词: Body Temperature; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Bronchoscopy; Cell Count; Cell Survival; Clinical Research; Collection; Computer Retrieval of Information on Scientific Projects Database; Data; Diagnosis; Diagnostic; Exhibits; Flow Cytometry; Funding; Grant; Heart Rate; Institution; Liquid substance; Macaca mulatta; Methods; Modification; Oxygen; Procedures; Purpose; Research; Research Personnel; Resources; Sampling; Source; Statistically Significant; Techniques; Temperature; United States National Institutes of Health; Variant; clinically relevant; nonhuman primate; rectal; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Bronchoalveolar lavage (BAL) via bronchoscopy is a diagnostic tool frequently used for clinical and research purposes in nonhuman primates. Although many institutions utilize this procedure, the technique is not standardized. One technical variant of the procedure is the method in which fluid is recovered. The purpose of this study was to evaluate differences between two different BAL aspiration techniques. Methods: Bronchoscopy and BAL fluid collection were performed on 20 rhesus macaques (Macaca mulatta). Data collected for comparison included heart rate, oxygen saturation levels, rectal temperature, volume of returned fluid, total cell count, cell viability, differential cell count, and flow cytometry. Results: Results showed no significant differences in the heart rate, SpO2 levels or body temperature between the two groups. Likewise, there were no statistical differences seen between the differential cell counts or the cell viability studies of the retrieved fluid using either method. When using the modified aspiration technique, an 8.3% increase in full fluid yield and a higher number of cells per microliter of fluid recovered were exhibited. Conclusion: The differences noted with the enhanced BAL technique are statistically significant and indicate a clinical relevance where diagnoses are concerned.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 背景：通过支气管镜进行支气管肺泡灌洗（BAL）是一种常用于非人类灵长类动物临床和研究目的的诊断工具。 尽管许多机构都使用此程序，但该技术并未标准化。 该程序的一种技术变体是回收液体的方法。本研究的目的是评估两种不同的 BAL 抽吸技术之间的差异。方法：对 20 只恒河猴（Macaca mulatta）进行支气管镜检查和支气管肺泡灌洗液采集。 收集用于比较的数据包括心率、氧饱和度水平、直肠温度、回流液量、总细胞计数、细胞活力、分类细胞计数和流式细胞术。结果：结果显示两组之间的心率、SpO2 水平或体温没有显着差异。 同样，使用这两种方法对回收的液体进行的差异细胞计数或细胞活力研究之间也没有发现统计学差异。 当使用改进的抽吸技术时，全液体产量增加了 8.3%，并且每微升回收液体的细胞数量增加。 结论：增强型 BAL 技术所观察到的差异具有统计学意义，并且表明与诊断相关的临床相关性。