MODIFICATION OF A COMMON BAL TECHNIQUE TO ENHANCE SAMPLE DIAGNOSTIC VALUE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景资料：经支气管镜进行支气管肺泡灌洗（BAL）是一种经常用于非人灵长类动物临床和研究目的的诊断工具。 虽然许多机构采用这一程序，但技术并不标准化。 该程序的一个技术变体是回收流体的方法。本研究的目的是评价两种不同BAL抽吸技术之间的差异。方法：对20只恒河猴进行支气管镜检查和支气管肺泡灌洗液收集。 收集用于比较的数据包括心率、氧饱和度水平、直肠温度、回流液体积、总细胞计数、细胞活力、细胞分类计数和流式细胞术。结果：两组患者的心率、SpO 2水平和体温无显著差异。 同样，使用两种方法回收的液体的细胞分类计数或细胞活力研究之间也没有观察到统计学差异。 当使用改进的抽吸技术时，显示出全流体产率增加8.3%，并且每微升回收的流体中细胞数量更高。 结论：使用增强BAL技术观察到的差异具有统计学显著性，并表明与诊断相关的临床相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Bronchoalveolar lavage (BAL) via bronchoscopy is a diagnostic tool frequently used for clinical and research purposes in nonhuman primates. Although many institutions utilize this procedure, the technique is not standardized. One technical variant of the procedure is the method in which fluid is recovered. The purpose of this study was to evaluate differences between two different BAL aspiration techniques. Methods: Bronchoscopy and BAL fluid collection were performed on 20 rhesus macaques (Macaca mulatta). Data collected for comparison included heart rate, oxygen saturation levels, rectal temperature, volume of returned fluid, total cell count, cell viability, differential cell count, and flow cytometry. Results: Results showed no significant differences in the heart rate, SpO2 levels or body temperature between the two groups. Likewise, there were no statistical differences seen between the differential cell counts or the cell viability studies of the retrieved fluid using either method. When using the modified aspiration technique, an 8.3% increase in full fluid yield and a higher number of cells per microliter of fluid recovered were exhibited. Conclusion: The differences noted with the enhanced BAL technique are statistically significant and indicate a clinical relevance where diagnoses are concerned.