BRONCHOALVEOLAR LAVAGE AND BRUSHINGS IN NORMALS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在这项研究中，将对正常志愿者进行支气管肺泡灌洗，并将结果与患者群体的数据进行比较。受试者在支气管镜检查前进行筛查。纤维支气管镜检查、支气管肺泡灌洗和支气管刷检将按标准化程序进行。BAL细胞将被量化，并进行细胞分化。实验室用于：血液分离、BAL分析、酶联免疫吸附试验、Northern分析、聚合酶链式反应、RNA提取、超速离心法、寡核苷酸合成、重组DNA技术和层流罩的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study bronchoalveolar lavage will be performed on normal volunteers and the results will be compared with the data from patient populations. Subjects are screened prior to bronchoscopy. Fiberoptic bronchoscopy, bronchoalveolar lavage and bronchial brushing will be performed according to standardized procedures. The BAL cells will be quantified and a cell differential made. The lab was used for the following: blood separation, BAL analysis, ELISA, Northern analysis, PCR, RNA isolation, ultracentrifugation, oligonucleotide synthesis, recombinant DNA techniques, and use of laminar flow hoods.