BRONCHOALVEOLAR LAVAGE IN ASBESTOS EXPOSED INDIVIDUALS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究将确定在与石棉暴露相一致的放射学异常的个人中，是否存在激活的肺泡巨噬细胞数量增加，这些细胞释放更多的肽生长因子，信号间质细胞增殖。研究人员将在mRNA水平上测量巨噬细胞生长因子以评估基因表达，并测量巨噬细胞上清液以定量自发蛋白释放。该实验室用于：BAL分析、RNA提取、超速离心法、重组DNA技术、血液分离、ELISA法和层流罩的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study will determine if in individuals with radiographic abnormalties consistent with their asbestos exposure, there are increased numbers of activated alveolar macrophages which release increased quantities of peptide growth factors that signal mesenchymal cells to proliferate. The investigators will measure macrophage growth factors at the mRNA level to evaluate gene expression and macrophage supernatants to quantitate spontaneous protein release. The lab was used for the following: BAL analysis, RNA Isolation, ultracentrifugation, recombinant DNA techniques, blood separation, ELISA and use of laminar flow hoods.