BRONCHOALVEOLAR LAVAGE AND BRUSHINGS IN NORMALS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study bronchoalveolar lavage will be performed on normal volunteers and the results will be compared with the data from patient populations. Subjects are screened prior to bronchoscopy. Fiberoptic bronchoscopy, bronchoalveolar lavage and bronchial brushing will be performed according to standardized procedures. The BAL cells will be quantified and a cell differential made. The lab was used for the following: blood separation, BAL analysis, ELISA, Northern analysis, PCR, RNA isolation, ultracentrifugation, oligonucleotide synthesis, recombinant DNA techniques, and use of laminar flow hoods.