BRONCHOALVEOLAR LAVAGE AND BRUSHINGS IN NORMALS

正常情况下的支气管肺泡灌洗和刷洗

• 批准号: 7605674
• 负责人: JOAN REIBMAN
• 金额: $ 0.83万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605674
• 关键词: Blood; Bronchial Lavages; Bronchoalveolar Lavage; Bronchoscopy; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Enzyme-Linked Immunosorbent Assay; Funding; Grant; Institution; Oligonucleotides; Patients; Polymerase Chain Reaction; Population; Procedures; RNA; Recombinant DNA; Research; Research Personnel; Resources; Source; Techniques; Ultracentrifugation; United States National Institutes of Health; volunteer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study bronchoalveolar lavage will be performed on normal volunteers and the results will be compared with the data from patient populations. Subjects are screened prior to bronchoscopy. Fiberoptic bronchoscopy, bronchoalveolar lavage and bronchial brushing will be performed according to standardized procedures. The BAL cells will be quantified and a cell differential made. The lab was used for the following: blood separation, BAL analysis, ELISA, Northern analysis, PCR, RNA isolation, ultracentrifugation, oligonucleotide synthesis, recombinant DNA techniques, and use of laminar flow hoods.

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在本研究中，将对正常志愿者进行支气管肺泡灌洗，并将结果与患者人群的数据进行比较。 在支气管镜检查前筛选受试者。 将根据标准化程序进行纤维支气管镜检查、支气管肺泡灌洗和支气管刷检。 将对BAL细胞进行定量并进行细胞分类。 该实验室用于：血液分离、BAL分析、ELISA、北方分析、PCR、RNA分离、超离心、寡核苷酸合成、重组DNA技术和层流罩的使用。