DEVELOPMENT OF REVERSIBLE INACTIVATION TECHNIQUE STUDY HIGHER COGNITIVE FUNCTION

可逆失活技术的开发研究高级认知功能

• 批准号: 7715732
• 负责人: JOCELYNE H BACHEVALIER
• 金额: $ 3.56万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715732
• 关键词: Adult; Animals; Anterior; Arts; Behavioral; Brain scan; Cannulas; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Funding; GABA Agonists; Grant; Hippocampus (Brain); Hour; Individual; Institution; Lesion; MRI Scans; Measures; Medial; Memory; Methods; Monkeys; Muscimol; Operative Surgical Procedures; Positioning Attribute; Procedures; Research; Research Personnel; Resources; Secure; Site; Source; Structure; Techniques; Temporal Lobe; Testing; Time; Training; United States National Institutes of Health; bone; cognitive function; implantation; male; neural circuit; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In our studies we used state-of-the-art reversible inactivation techniques to probe in greater detail the interactions between different components of the medial temporal lobe in memory functions in monkeys. These methods permit individual structures within the medial temporal lobe to be selectively turned "off" and "on" for a short period of time while some specific memory functions are tested. These reversible inactivation procedures create "virtual lesions" as the underlying neural circuitry is not structurally disrupted and a particular site is only temporarily deactivated. In the current year, one adult male monkey received a presurgical MRI scan of the brain to determine the coordinate sites for our targeted structures (the hippocampi). Then, surgical implantation of two guide cannulae positioned at the anterior and posterior portions of each hippocampus and secured on the bone. This animal is currently behaviorally trained to measure spatial memory abilities. In half of the testing sessions the target structure is inactivated with muscimol (GABA agonist), and with the other half, vehicle is injected. Therefore each animal is used as its own control. The preliminary data indicate that muscimol achieved maximal behavioral effect between 25 minutes to one hour, after which the effects begin to wear off.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在我们的研究中，我们使用了最先进的可逆失活技术，更详细地探讨了猴子记忆功能中内侧颞叶不同组成部分之间的相互作用。这些方法允许内侧颞叶内的单个结构在短时间内选择性地“关闭”和“打开”，同时测试某些特定的记忆功能。 这些可逆的失活过程产生“虚拟损伤”，因为潜在的神经回路在结构上没有被破坏，并且特定的位点只是暂时失活。 在今年，一只成年雄性猴子接受了术前脑部MRI扫描，以确定我们的目标结构（大脑皮层）的坐标位置。 然后，在每个海马的前部和后部手术植入两个引导套管，并固定在骨上。 这种动物目前正在接受行为训练，以测量空间记忆能力。 在一半的测试阶段中，用蝇蕈醇（GABA激动剂）灭活靶结构，另一半注射载体。 因此，每只动物用作其自身对照。 初步数据表明蝇蕈醇在25分钟至1小时之间达到最大行为效应，之后效应开始消失。