OPTIMIZED EMBRYONIC STEM CELL CULTURE MEDIA

优化的胚胎干细胞培养介质

• 批准号: 7716407
• 负责人: James Alexander Thomson
• 金额: $ 8.19万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-23 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716407
• 关键词: Applications Grants; Biological Assay; Budgets; Cell Line; Cell Survival; Cellular Morphology; Competence; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Data; Drug Formulations; Environment; Funding; Future; Gases; Goals; Grant; Individual; Institution; Location; Methods; Osmolar Concentration; Protocols documentation; Rate; Research; Research Personnel; Resources; Screening procedure; Source; System; Time; United States National Institutes of Health; base; cost; embryonic stem cell; human embryonic stem cell; improved; self-renewal

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To develop a media formulation and protocol for the human ES cell culture that will improve human ES cell viability and promote uniformity, consistency and ease of use across a variety of users in different locations. Some of the specific objectives necessary to accomplish this goal are to: 1) optimize the physiochemical environment, 2) optimize the basal media formulation and 3) eliminate undefined media components. In this budget period, we were able to develop a both a short-term and intermediate screening method that will significantly reduce the cost and time required for future optimization studies. Using this new assay system we have already identified several factors that contribute to self-renewal of human ES cells in a defined culture systems. This data will serve as the basis for future grant applications. Additionally the physiochemical environment for human ES cells, including pH, osmolarity and gas amosphere, was optimized. Lastly more than 90 individual factors were screened for their effect on human ES cell competence (including cell morphology, proliferation and spontaneous differentiation rates) This research used WNPRC resources and federally approved hES cell lines.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 开发用于人ES细胞培养的培养基配方和方案，以提高人ES细胞活力，并促进不同地点的各种用户的均匀性、一致性和易用性。实现这一目标所需的一些具体目标是：1）优化理化环境，2）优化基础培养基配方，3）消除不确定的培养基成分。 在本预算期内，我们能够开发一种短期和中期筛选方法，这将大大减少未来优化研究所需的成本和时间。 使用这种新的检测系统，我们已经确定了几个因素，有助于自我更新的人ES细胞在一个确定的培养系统。 这些数据将作为今后赠款申请的基础。此外，对ES细胞的理化环境，包括pH值、渗透压和无气环境进行了优化。最后筛选了90多个因素对人ES细胞活性（包括细胞形态、增殖和自发分化率）的影响。