INHALATION EXPOSURE FACILITY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 目的：位于CNPRC的吸入暴露设施是大学校园中最大的设施之一。 它允许使用非人灵长类动物进行独特的人类健康相关肺部研究的机会。 有能力在体内或体外暴露于精确表征和控制的气体和气溶胶气氛。 就空气污染对健康的影响研究而言，用于接触研究的试验对象范围可包括动物、分离和灌注的肺、气管外植体和人或猴肺细胞培养物。 这允许一个综合的，比较的方法来定义呼吸系统损伤和修复的机制。 一个相对较新的能力是肺功能实验室，提供了一个全面的测试，从婴儿到成年非人灵长类动物。 提供的服务： + 特殊暴露 + 臭氧生成和监测 + 气溶胶生成和分析 + 氮氧化物生成和监测 + 过敏原的产生和分析 + 过滤空气暴露 + ETS生成和分析 肺功能检测实验室 + 基线气道阻力测试 + 气道反应性测试 + 过敏原反应性试验 + 静态肺力学测试 + 晚期过敏原反应性试验 该设施继续提供稳定的、充分表征的暴露于空气污染物、过敏原、治疗剂、气溶胶和所支持的研究项目所需的其他测试气氛的暴露。 作为核心服务提供者，该设施在本报告所述期间支助了13个研究项目，约有34名主要调查员。 除了加州大学戴维斯分校的研究人员外，还向以下机构的科学家提供了核心服务：亚拉巴马大学伯明翰分校、密歇根州立大学、宾夕法尼亚州立大学、路易斯安那州立大学、化学工业毒理学研究所、美国环保署、西北太平洋国家实验室、国家犹太医学和研究中心、加州大学旧金山分校医学院、Innate Pharma和Genentech，Inc.。 研究活动的主要重点是进一步研究非人类灵长类动物的哮喘和空气污染的吸入暴露。 对提供过滤空气的新型模块化动物舍进行了测试，并投入常规使用。 获得了用于通过支气管镜向恒河猴微喷雾递送溶液和悬浮液，特别是抗体的设备。 其他非人类灵长类项目包括使用间接量热法进行持续的代谢测量。 正在开发一种新的系统，用于生成燃烧产物，包括多环芳烃，用于吸入室研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The Inhalation Exposure Facility located at the CNPRC is one of the largest in existence on a university campus. It permits unique human health-related pulmonary research opportunities using nonhuman primates. Capabilities exist for in vivo or in vitro exposure to precisely characterized and controlled atmospheres of gases and aerosols. For health effects of air pollution research, the range of test subjects used for exposure studies can include animals, isolated and perfused lungs, tracheal explants and human or monkey lung cell cultures. This permits an integrated, comparative approach to defining mechanisms of respiratory system injury and repair. A relatively recent addition to the capabilities is a pulmonary function laboratory that offers a comprehensive array of testing for infant through adult nonhuman primates. Services Offered: + Special Exposures + Ozone Generation and Monitoring + Aerosol Generation and Analysis + NOx Generation and Monitoring + Allergen Generation and Analysis + Filtered Air Exposure + ETS Generation and Analysis Pulmonary Function Testing Laboratory + Baseline Airway Resistance Testing + Airway Responsiveness Testing + Allergen Responsiveness Testing + Static Lung Mechanics Testing + Late Phase Allergen Responsiveness Testing This facility continued to provide stable, well characterized exposures to air pollutants, allergens, therapeutic agents, aerosols and other test atmospheres as required by the research projects supported. As a Core service provider, the facility supported 13 research projects during the reporting period with some 34 major investigators. In addition to UC Davis investigators, core services were provided to scientists at University of Alabama at Birmingham, Michigan State University, Pennsylvania State University, Louisiana State University, Chemical Industry Institute of Toxicology, U.S. EPA, Pacific Northwest National Laboratory, National Jewish Medical and Research Center, UCSF School of Medicine, Innate Pharma and Genentech, Inc. Major emphasis in research activities was directed to further inhalation exposure studies of asthma and air pollution in nonhuman primates. New modular building animal housing supplied with filtered air was tested and put into routine use. Equipment was obtained for microspray delivery of solutions and suspensions, and particularly antibodies, through a bronchoscope to rhesus macaques. Other nonhuman primate projects included continuing metabolic measurements using indirect calorimetry. A new system is under development for the generation of combustion products including polycyclic aromatic hydrocarbons for inhalation chamber studies.