HOST DNA METHYLATION PATTERNS IN ASSOCIATION WITH PERIODONTAL DISEASE

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose: The overall goal of this research is to identify changes in gene expression and alterations of DNA methylation status in subjects who exhibit advanced chronic periodontal inflammation. Participants: Thirty subjects will be recruited from Graduate Periodontics Clinic and Dental Faculty Practice at the University of North Carolina School of Dentistry. Fifteen subjects will exhibit advanced chronic periodontitis (diseased), and 15 will exhibit periodontal health (healthy). Procedures (methods): The following procedures will be performed during the visit(s), with the restriction that all biological specimens will be collected on the same day, or that all biological specimens except the biopsy (ies) will be taken on one day and the biopsy (ies) on the next day: an oral and periodontal screening exam, approximately 40 ml of whole blood will be collected via venipuncture, collection of 16 gingival crevicular fluid samples, collection of subgingival plaque sample(s), all teeth will be scored for pocket depth, percent bleeding on probing and clinical attachment level unless the patient s dental school chart has these recent (within six months) measurements. In addition, one gingival biopsy sample per healthy subject will be collected at the time of a routine crown extension procedure, and two gingival biopsy samples will be obtained from periodontitis subjects at the time of a periodontal flap procedure. All biopsy samples are tissues that would otherwise be discarded during the dental procedures.