HOST DNA METHYLATION PATTERNS IN ASSOCIATION WITH PERIODONTAL DISEASE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：这项研究的总体目标是确定在基因表达的变化和DNA甲基化状态的改变，在受试者谁表现出先进的慢性牙周炎。 参会人员：将从北卡罗来纳州大学牙科学院的研究生牙周病诊所和牙科学院实践中招募30名受试者。15名受试者将表现出晚期慢性牙周炎（患病），15名将表现出牙周健康（健康）。 程序（方法）：访视期间将进行以下程序，限制条件是所有生物样本将在同一天采集，或者除活检外的所有生物样本将在一天采集，活检在第二天采集：口腔和牙周筛查检查，将通过静脉穿刺收集约40 ml全血，收集16个龈沟液样本， 收集龈下菌斑样本时，将对所有牙齿的牙周袋深度、探诊出血百分比和临床附着水平进行评分，除非患者的牙科学校图表最近（六个月内）有这些测量结果。此外，每例患者的牙龈活检样本 健康受试者将在常规牙冠延长程序时被收集，并且两个牙龈活组织检查样品将在牙周瓣程序时从牙周炎受试者获得。所有活检样本都是在牙科手术期间会被丢弃的组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose: The overall goal of this research is to identify changes in gene expression and alterations of DNA methylation status in subjects who exhibit advanced chronic periodontal inflammation. Participants: Thirty subjects will be recruited from Graduate Periodontics Clinic and Dental Faculty Practice at the University of North Carolina School of Dentistry. Fifteen subjects will exhibit advanced chronic periodontitis (diseased), and 15 will exhibit periodontal health (healthy). Procedures (methods): The following procedures will be performed during the visit(s), with the restriction that all biological specimens will be collected on the same day, or that all biological specimens except the biopsy (ies) will be taken on one day and the biopsy (ies) on the next day: an oral and periodontal screening exam, approximately 40 ml of whole blood will be collected via venipuncture, collection of 16 gingival crevicular fluid samples, collection of subgingival plaque sample(s), all teeth will be scored for pocket depth, percent bleeding on probing and clinical attachment level unless the patient s dental school chart has these recent (within six months) measurements. In addition, one gingival biopsy sample per healthy subject will be collected at the time of a routine crown extension procedure, and two gingival biopsy samples will be obtained from periodontitis subjects at the time of a periodontal flap procedure. All biopsy samples are tissues that would otherwise be discarded during the dental procedures.