HOST DNA METHYLATION PATTERNS IN ASSOCIATION WITH PERIODONTAL DISEASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：这项研究的总体目标是确定晚期慢性牙周炎患者的基因表达变化和DNA甲基化状态的变化。 研究对象：来自北卡罗来纳大学牙学院牙周科诊所和牙科专业实习的30名受试者。15名受试者将表现为晚期慢性牙周炎(患病)，15名受试者将表现为牙周健康(健康)。 程序(方法)：在访问期间进行以下程序(S)，限制在同一天采集所有生物标本，或除活检外的所有生物标本在第一天采集，第二天进行活检：口腔和牙周筛查，通过静脉穿刺法采集全血约40ml，采集16例牙周沟液标本， 根据收集的牙菌斑样本(S)，所有牙齿都将根据牙袋深度、探诊出血百分比和临床附着水平进行评分，除非患者S的牙科学校图表有这些最近(在六个月内)的测量结果。此外，每个人都有一份牙龈活检样本 健康受试者将在常规加冠手术时采集，牙周炎受试者在牙周翻瓣手术时将获得两个牙龈活检样本。所有的活检样本都是在牙科手术过程中会被丢弃的组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose: The overall goal of this research is to identify changes in gene expression and alterations of DNA methylation status in subjects who exhibit advanced chronic periodontal inflammation. Participants: Thirty subjects will be recruited from Graduate Periodontics Clinic and Dental Faculty Practice at the University of North Carolina School of Dentistry. Fifteen subjects will exhibit advanced chronic periodontitis (diseased), and 15 will exhibit periodontal health (healthy). Procedures (methods): The following procedures will be performed during the visit(s), with the restriction that all biological specimens will be collected on the same day, or that all biological specimens except the biopsy (ies) will be taken on one day and the biopsy (ies) on the next day: an oral and periodontal screening exam, approximately 40 ml of whole blood will be collected via venipuncture, collection of 16 gingival crevicular fluid samples, collection of subgingival plaque sample(s), all teeth will be scored for pocket depth, percent bleeding on probing and clinical attachment level unless the patient s dental school chart has these recent (within six months) measurements. In addition, one gingival biopsy sample per healthy subject will be collected at the time of a routine crown extension procedure, and two gingival biopsy samples will be obtained from periodontitis subjects at the time of a periodontal flap procedure. All biopsy samples are tissues that would otherwise be discarded during the dental procedures.