Adaptive Optics Instrumentation for Advanced Ophthalmic Imaging

用于高级眼科成像的自适应光学仪器

• 批准号: 7931934
• 负责人: DAVID R WILLIAMS
• 金额: $ 101.25万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7931934
• 关键词: Affect; Age related macular degeneration; Axon; Biological Markers; Biomedical Engineering; Blood capillaries; Burn injury; Cells; Clinic; Clinical Research; Clinical Trials; Computer software; Computers and Advanced Instrumentation; Dendrites; Detection; Development; Devices; Disease; Disease Progression; Early Diagnosis; Engineering; Environment; Eye; Eye Movements; Fluorescence; Fluorescent Dyes; Fluorescent Probes; Functional disorder; Funding; Future; Generations; Genetic Markers; Genotype; Glaucoma; Grant; Human; Hybrids; Image; Imagery; Indiana; Individual; Inherited; International; Label; Laboratories; Lasers; Lead; Leukocytes; Life; Light; Macular degeneration; Measures; Methods; Microscopic; Monkeys; Montana; Motion; Mus; Ophthalmoscopes; Ophthalmoscopy; Optics; Patients; Phase; Phenotype; Photoreceptors; Principal Investigator; Rattus; Research; Research Personnel; Resolution; Retina; Retinal; Retinal Cone; Retinal Degeneration; Retinal Diseases; Rodent; Scanning; Signal Transduction; Structure; System; Techniques; Technology; Time; Treatment Efficacy; Universities; adaptive optics; austin; capillary; cellular imaging; coping; design; disorder of macula of retina; ergonomics; fluorescence imaging; ganglion cell; image processing; in vivo; inherited retinal degeneration; innovation; instrument; neuronal cell body; next generation; physical science; public health relevance; relating to nervous system; response; skills; software development; tool; vision science

DESCRIPTION (provided by applicant): This Bioengineering Research Partnership is a consortium of 6 laboratories that are building adaptive optics scanning laser ophthalmoscopes (AOSLOs) and applying them to microscopic examination of the living normal and diseased retina. The principal investigator is David Williams (University of Rochester) who introduced the first successful adaptive optics instruments to vision science. Other lead investigators include: Steve Burns (Indiana University) an international leader in laser scanning ophthalmoscopy, John Flannery (UC, Berkeley) an expert in retinal degeneration and the development of retinal biomarkers, and Austin Roorda (UC, Berkeley) who designed the first adaptive optics scanning laser ophthalmoscopes, David Arathorn (Montana State University) who brings strong mathematical skills and software development tools for tracking the eye in AOSLOs, and R. Daniel Ferguson (Physical Sciences, Inc.) whose expertise is in the optical engineering of innovative eye tracking systems. During years 1-5 of the previous funding period, the partnership designed and built four AOSLO instruments and two more instruments are under construction. These devices have produced the first images ever of numerous microscopic structures in the living eye including the RPE cell mosaic, single leucocytes flowing in the smallest retinal capillaries, and fluorescently-labelled ganglion cell dendrites, axons and cell bodies. In addition, technical challenges for imaging eyes ranging in size from human to rodent have been overcome. The partnership is now proposing continued funding for years 6-10 to develop new capabilities for these instruments such as a combined hardware and software approach to reduce the effects of eye motion on high resolution retinal imagery. We also will develop a new generation of instruments with special capabilities, such as the ability to image ganglion cells in the living human eye without the use of fluorescent dyes, and the ability to optically record neural responses from specific retinal cells. PUBLIC HEALTH RELEVANCE: This application will develop a technology, adaptive optics scanning laser ophthalmoscopy, for taking extremely sharp pictures of the inside of the living eye, so sharp that individual cells can be seen. This technology will be used to study diseases such as age-related macular degeneration and glaucoma. It may allow the earlier detection of retinal disease, better tracking of disease progression, and the efficacy of therapies for retinal disease.

描述（由申请人提供）：这种生物工程研究合作伙伴关系是由6个实验室组成的联盟，这些实验室正在建造自适应光学扫描激光眼镜（Aoslos），并将其应用于对生活正常和疾病的视网膜的微观检查。主要研究人员是罗切斯特大学的戴维·威廉姆斯（David Williams），他将第一个成功的自适应光学工具引入了视力科学。其他主要研究人员包括：史蒂夫·伯恩斯（Indiana University）是激光扫描眼镜检查的国际领导者，约翰·弗兰纳里（John Flannery）（UC，伯克利UC，伯克利）是视网膜退化和视网膜生物标志物的发展专家带来了强大的数学技能和软件开发工具，用于跟踪Aoslos的眼睛，以及R. Daniel Ferguson（物理科学，Inc。），其专业知识是在创新的眼动追踪系统的光学工程中。在上一个融资期的1 - 5年中，合作伙伴关系设计和建造了四种AOSLO工具和另外两种工具正在建设中。这些设备在活眼中产生了有史以来许多显微镜结构的第一批图像，包括RPE细胞镶嵌物，最小的视网膜毛细血管中流动的单白细胞以及荧光贴标记的神经节细胞树突，轴突和细胞体。此外，已经克服了从人到啮齿动物的大小的成像眼睛的技术挑战。该合作伙伴关系现在提议在6 - 10年内持续资助，以开发这些工具的新功能，例如合并的硬件和软件方法，以减少眼动对高分辨率视网膜图像的影响。我们还将开发具有特殊功能的新一代仪器，例如无需使用荧光染料而在生命的人眼中成像神经节细胞的能力，以及能够通过特定视网膜细胞的神经反应进行光学记录的能力。公共卫生相关性：该应用程序将开发一种技术，自适应光学扫描激光眼镜检查，用于拍摄活眼内部的极其清晰的照片，如此敏锐，以至于可以看到单个细胞。该技术将用于研究与年龄相关的黄斑变性和青光眼等疾病。它可以允许早期发现视网膜疾病，更好地跟踪疾病进展以及治疗性视网膜疾病的功效。