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High Resolution Mapping of Foveal Ganglion Cell Receptive Fields in the Living Primate Eye

活体灵长类动物眼睛中心凹神经节细胞感受野的高分辨率绘图

基本信息

批准号：
10319191
负责人：
DAVID R WILLIAMS
金额：
$ 52.47万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-01 至 2025-11-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10319191
关键词：
Anatomy Brain Calcium Calcium Signaling Cells Classification Code Color Color Visions Complement Complex Cone Development Disease Electrophysiology (science)Eye Floods Fluorescence Funding Grain Histologic Histology Human Image Imaging Techniques In Vitro Individual Injury Investigation Knowledge Laboratories Light Location Maps Mediating Methods Monkeys Morphology Mus Noise Ophthalmoscopes Performance Peripheral Photoreceptors Physiological Physiology Primates Property Resolution Retina Retinal Cone Retinal Ganglion Cells Scanning Signal Transduction Stimulus Structure Surveys System Techniques Time Vision Yin adaptive optics arm blind calcium indicator design flexibility fluorescence imaging fovea centralis ganglion cell improved in vivo instrument melanopsin novel novel strategies receptive field reconstruction response retinal imaging sight restoration spatial vision spatiotemporal visual stimulus

项目摘要

The importance for vision of the tiny fovea has been established by centuries of investigation as well as observations of the devastating consequences of its damage through injury or disease. Though evidence suggests that the fovea contains the full complement of the two dozen or so classes of ganglion cells found in peripheral retina, we know little about the physiology of these foveal cells. This gap in our understanding is the result of challenges in obtaining electrophysiological recordings from this delicate and topographically-complex structure. These challenges have been overcome by a method developed in our laboratory that allows simultaneous calcium imaging of the fluorescence responses of hundreds of foveal retinal ganglion cells in response to visual stimuli. Because this technique allows recording from single cells without damage in the living eye, we can study the same cells for months or even years, offering the opportunity to characterize the performance of each cell more thoroughly than has been possible with any prior method. Since the first submission of the proposal, we have made significant improvements in the expression of calcium indicator, GCamMP6s, in ganglion cells that increases the extent of expression to greater eccentricities, the fluorescence signal from each cell, as well as reducing the loss of ganglion cells over time. Moreover, we have designed a new ophthalmoscope with two independent adaptive optics systems, one dedicated to high resolution stimulus delivery and a second dedicated to high resolution ganglion cell recording. We have also developed an extensive battery of visual stimuli to characterize the responses of each cell in space, time, and color. This battery will include a white noise stimulus capable of identifying the locations and classes of single cone inputs to the receptive fields of foveal ganglion cells. To assist in cell classification, these physiological observations will be supplemented with ex vivo and in vivo histological analysis of the morphology of ganglion cell dendritic arbors. Armed with these improvements, we will undertake a comprehensive survey of both the physiology and anatomy of the foveal ganglion cell classes that mediate primate foveal vision.

几个世纪以来的研究已经证实了小中央凹对视力的重要性