Core C: Genomics and High Throughput Screening Core

核心 C：基因组学和高通量筛选核心

• 批准号: 8066104
• 负责人: SCOTT L DIAMOND
• 金额: $ 25.95万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8066104
• 关键词: Core; Genomics; Throughput; Screening

This Core provides cutting edge tools that allow invesfigators to monitor cellular and molecular changes that occur during normal hematopoiesis or in pathological states associated with bone marrow failure (BMF), nonmalignant myelodysplastic syndromes or defects in erythropoiesis/megakaryopoiesis, addressing the three foci of this application as detailed in the OVERALL DESCRIPTION. There is a clear need for services to identify and analyze in a rapid, high throughput fashion drugs that influence hematopoiefic cell commitment to various lineages and to develop miniaturized systems for studies of stromal/niche environments. Dr. Diamond is the director of the University of Pennsylvania (UPENN) PCMD, which he established as a Core Facility in 2005 (see BIOSKETCH). Dr. Diamond has 20 years of experience with numerous projects in endothelial biology, blood biology, and blood systems biology. He already has been working with Drs. Poncz, French and Gadue on niche effects on thrombopoiesis from mature megakaryocytes. He will be overall Director and Director of SubCore C-1. Dr. Baldwin will direct the analysis of RNA and DNA changes in cells during hematopoiesis. He has 11 years of industrial and academic experience in microarray assays, serves on the Neuroscience Microarray Consortium Advisory Panel for the NIH, co-chairs the MicroArray Research Group of the Associafion of Biomolecular Resource Facilifies, and interacts regularly with core directors on a nafional level (see BIOSKETCH). Dr. Baldwin founded the Penn Microarray Facility in 2001, was appointed Director of the Molecular Diagnosis and Genotyping Facility in 2005, and has merged the two laboratories to create the Molecular Profiling Facility. He has conducted previous genomic profiling projects with Drs. Weiss, Blobel, Carroll, Diamond, Discher, Gewirtz, June and Pear on efforts directly relevant to this P30. We have combined components of the two UPENN cores run by Drs. Diamond and Baldwin to develop a single core for this P30 to incorporate state-of-the-art resources on our campus that will unite our abilities to analyze normal, pathologic and manipulated hematopoietic cells. SubCore C-1 offers high-throughput microscale screening for compounds that affect various aspects of hematopoiefic cell biology, including lineage commitment (either from embryonic stem (ES) cells or more differentiated progenitors), survival, proliferation or maturafion into mature blood cells. SubCore C-2 will analyze changes that occur in the RNA expression profiles and epigenetic DNA marks in these cells either de novo or after modifications using Core E. We believe that these two SubCores reflect a common theme of rapidly analyzing cells and cellular changes using complex methodologies with significant investment in rapidly changing equipment and technologies. Core C is committed to bringing the most current and innovative technologies to the P30 investigators, while providing campus-wide standardization of services to support cooperafive efforts by different invesfigators with complementary interests and expertise. Our goal is to provide significant labor-intensive service and guidance at very compefitive pricing, supported in part by efficiency of utilizafion and by UPENN financial support.

该核心提供了尖端工具，使入侵器能够监测正常造血作用或与骨髓衰竭（BMF），非质量骨髓异常综合征或缺陷相关的病理状态或病理状态中发生的细胞和分子变化，或者在脑中/巨麦卡里奥波伊西斯（Megakaryopoiesis）中进行了详细描述，该焦点是详细描述的三个焦点。显然需要服务来识别和 在快速，高通量的时尚药物中分析，影响造血细胞对各种谱系的承诺，并开发用于基质/利基环境研究的小型化系统。 Diamond博士是宾夕法尼亚大学（UPENN）PCMD的主任，他于2005年建立为核心设施（请参阅Biosketch）。 Diamond博士在内皮生物学的众多项目中拥有20年的经验 生物学和血液系统生物学。他已经与Drs合作。 Poncz，法语和Gadue对成熟巨核细胞对血小板的利基影响。他将担任Subcore C-1的总监和总监。鲍德温博士将指导造血过程中细胞中RNA和DNA变化的分析。他在微阵列分析方面拥有11年的工业和学术经验，在NIH的神经科学微阵列联盟咨询小组中，共同主席生物分子资源协会的微阵列研究小组会促进，并与核心级别的核心导演互动 （请参阅Biosketch）。鲍德温博士于2001年成立了Penn微阵列设施，于2005年被任命为分子诊断和基因分型设施的主任，并合并了两个实验室来创建分子分析设施。他曾与DRS进行了以前的基因组分析项目。 Weiss，Blobel，Carroll，Diamond，Discher，Gewirtz，June和Pear与此P30直接相关的努力。 我们结合了由Drs运行的两个Upenn核心的组件。钻石和鲍德温（Diamond）和鲍德温（Baldwin）为此P30开发一个核心，以在我们的校园中纳入最先进的资源，以团结我们的能力，以分析正常，病理和操纵的造血细胞。子核C-1提供了影响血小蛋白细胞生物学各个方面的化合物的高通量显微镜筛选，包括谱系承诺（来自胚胎茎（ES）细胞（ES）细胞或更多分化的祖细胞），生存，增生或成熟的血液细胞。子核C-2将分析这些细胞中RNA表达谱和表观遗传DNA标记中发生的变化或使用核心E进行修改后的表观遗传学标记。我们相信，这两个子核反映了在快速更换设备和技术中具有重大投资的大量投资的复杂方法快速分析细胞和细胞变化的共同主题。 Core C致力于将最新和创新的技术带给P30调查人员，同时提供范围内的服务标准化，以支持具有互补利益和专业知识的不同Invesfigators的库存努力。我们的目标是在非常有利的定价中提供大量劳动密集型服务和指导，部分由Utilizafion的效率和Upenn Fanancial Support提供支持。