Genomics and Ultra-High-Throughput Sequencing Core

了解在衰老过程中控制成体干细胞稳态的调控网络是关键的一步。 发现在生命中保存不同组织功能的方法。超高吞吐量 测序方法将允许在全基因组范围内对转录调控因子进行无偏见的识别 老化干细胞中的结合部位和组蛋白修饰模式。这一知识将对破译 在成年生物体中维持干细胞储存库的分子机制。世界银行的使命是 基因组学和超高通量测序的核心将是： 1.为了产生一致的转录因子结合位点数据，染色质的表观遗传修饰， 以及可在计划项目的三个实验室之间共享的RNA成绩单； 2.为数据分析提供计算和统计支持，开发新的生物信息学 不同年龄的不同干细胞类型的数据集的联合分析方法； 3.建立和维护一个数据管理系统，以促进两国之间的数据交换 参与实验室。 相关性(请参阅说明)： 成人转录和表观遗传网络随年龄变化的无偏鉴定 干细胞将是决定这些细胞自始至终调控机制的关键。 生命，并将有助于发现新的途径，以抵消与年龄相关的组织再生下降。

Understanding the regulatory networks that control adult stem cell homeostasis during aging is a critical step in discovering ways to preserve the function of different tissues throughout lifespan. Ultra-high-Throughput sequencing approaches will allow the unbiased genome-wide identification of transcriptional regulator binding sites and histone modification patterns in aging stem cells. This knowledge will be pivotal to decipher the molecular mechanisms that maintain stem cell reservoirs in adult organisms. The mission of the Genomics and Ultra-High-Throughput Sequencing Core will be: 1. To generate consistent data for transcription factor binding sites, epigenetic modifications on chromatin, and RNA transcripts that can be shared between the three laboratories of the Program Project; 2. To provide computational and statistical support for data analysis and to develop new bioinformatic approaches for the combined analysis of datasets from different stem cell types at different ages; 3. To establish and maintain a data management system that will facilitate data exchange between the participating laboratories. RELEVANCE (See instructions): The unbiased identification of age-dependent changes in transcriptional and epigenetic networks in adult stem cells will be pivotal for determining the mechanisms underlying the regulation of these cells throughout life, and will help uncover new avenues to counteract age-dependent decline in tissue regeneration.