Genomics and Ultra-High-Throughput Sequencing Core

了解在衰老过程中控制成体干细胞稳态的调节网络是关键的一步 发现了在整个生命周期中保持不同组织功能的方法。超高吞吐量 测序方法将允许无偏的全基因组鉴定转录调节因子 结合位点和组蛋白修饰模式。这些知识对于破解 维持成体干细胞库的分子机制。联合国的使命 基因组学和超高重复测序核心将是： 1.为了生成转录因子结合位点、染色质上的表观遗传修饰、 以及可以在计划项目的三个实验室之间共享的RNA转录本; 2.为数据分析提供计算和统计支持，并开发新的生物信息学 用于对来自不同年龄的不同干细胞类型的数据集进行组合分析的方法; 3.建立和维持一个数据管理系统，以促进 参与实验室。 相关性（参见说明）： 成年人转录和表观遗传网络中年龄依赖性变化的无偏鉴定 干细胞将是决定这些细胞调节机制的关键， 这将有助于发现新的途径来对抗组织再生中的年龄依赖性下降。

Understanding the regulatory networks that control adult stem cell homeostasis during aging is a critical step in discovering ways to preserve the function of different tissues throughout lifespan. Ultra-high-Throughput sequencing approaches will allow the unbiased genome-wide identification of transcriptional regulator binding sites and histone modification patterns in aging stem cells. This knowledge will be pivotal to decipher the molecular mechanisms that maintain stem cell reservoirs in adult organisms. The mission of the Genomics and Ultra-High-Throughput Sequencing Core will be: 1. To generate consistent data for transcription factor binding sites, epigenetic modifications on chromatin, and RNA transcripts that can be shared between the three laboratories of the Program Project; 2. To provide computational and statistical support for data analysis and to develop new bioinformatic approaches for the combined analysis of datasets from different stem cell types at different ages; 3. To establish and maintain a data management system that will facilitate data exchange between the participating laboratories. RELEVANCE (See instructions): The unbiased identification of age-dependent changes in transcriptional and epigenetic networks in adult stem cells will be pivotal for determining the mechanisms underlying the regulation of these cells throughout life, and will help uncover new avenues to counteract age-dependent decline in tissue regeneration.