The Interactions Core/Mark Mcintosh

交互核心/马克·麦金托什

• 批准号: 8007179
• 负责人: DENNIS B LUBAHN
• 金额: $ 22.51万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007179
• 关键词: Affect; Anti-Inflammatory Agents; Anti-inflammatory; Antioxidants; Biochemical; Bioinformatics; Biological; Biometry; Botanicals; Complement; Cysteine; DNA; Data; Data Set; Disease; Disease model; Erinaceidae; Estrogens; Funding; Gel; Gene Expression Regulation; Gene Proteins; Genome; Genomics; Goals; Human Resources; Informatics; Label; Libraries; Maps; Mass Spectrum Analysis; Measures; Messenger RNA; Methods; Modeling; Modification; Molecular; NADPH Oxidase; Output; Oxidation-Reduction; Pathway interactions; Plasma; Post-Translational Modification Site; Post-Translational Protein Processing; Process; Production; Proteins; Proteomics; Protocols documentation; Regulation; Relative (related person); Research Personnel; Research Project Grants; Route; Sampling; Scientist; Screening procedure; Signal Pathway; Signal Transduction; Site; Stress-Induced Protein; Sulfhydryl Compounds; Systems Biology; Technology; Transduction Gene; Validation; Work; base; instrument; network models; nitrosative stress; protein expression; response

The Interactions Core: Mega-sequencing, Proteomics, Informatics and Nitrosylation will provide proteomic analysis, mRNA mega-sequencing, bioinformatic analyses, and biostatistics to support the research projects. For proteomic analysis of antioxidant, anti-inflammatory, NADPH oxidase, hedgehog, and estrogen signaling pathway targets, affecting protein expression levels and redox-based protein post-translational modifications (PTM), particularly S-nitrosylation on specific protein cysteine thiols, we will collaborate with MU's Charles W. Gehrke Proteomics Center to develop an integrative proteomics strategy including both gel- and LC/MSbased quantitative proteomics. We will develop protein cysteine post-translational modification PTM site mapping using CID/ETD technology and provide our expertise to identify potential redox targets and aid in mechanistic studies. High-throughput mRNA mega-sequencing will also be completed by personnel in this core. We will work with the MU DNA Facility and its ultra-high throughput sequencing platform. Sequence data output will be routed directly to the bioinformatician for initial processing, library construction, and screening. In later years of the funding period, we will develop protocols to look at protein-level changes in plasma. Both genomic and proteomic data will be integrated into a pathway interaction model that will provide hypotheses for experimental validation. Overall the Core will provide support to the research projects and work to develop a deeper understanding of how botanicals can influence signaling pathways.

互动核心：大测序，蛋白质组学，信息学和亚硝基化将提供蛋白质组学 分析，mRNA大测序，生物信息学分析和生物统计学，以支持研究项目。 用于抗氧化、抗炎、NADPH氧化酶、hedgehog和雌激素信号传导的蛋白质组学分析 途径靶点，影响蛋白质表达水平和基于氧化还原的蛋白质翻译后修饰 (PTM)，特别是特定蛋白质半胱氨酸巯基上的S-亚硝基化，我们将与MU的Charles W. Gehrke蛋白质组学中心将开发一种整合的蛋白质组学策略，包括凝胶和LC/MS 定量蛋白质组学我们将开发蛋白半胱氨酸翻译后修饰PTM位点 利用CID/ETD技术进行测绘，并提供我们的专业知识来识别潜在的氧化还原目标， 机械研究。高通量mRNA大测序也将由本中心的人员完成。 核心我们将与MU DNA设施及其超高通量测序平台合作。序列 数据输出将直接发送给生物信息学家进行初始处理、文库构建， 筛选在资助期的后几年，我们将制定协议，以观察蛋白质水平的变化， 等离子体基因组和蛋白质组数据将被整合到一个途径相互作用模型中， 为实验验证提供假设。总体而言，核心将为研究项目提供支持 并致力于更深入地了解植物如何影响信号通路。