Botanical/Plant Genomics Core/Wendy Applequist

植物学/植物基因组学核心将通过提供一些植物测试材料，确保植物材料的身份和质量得到适当的记录，并为所研究物种的种下变异研究提供样品来支持研究项目。具体目标包括(1)识别、记录和提供植物原料；(2)收集、培育和供应接骨木材料，用于种下变异研究；（三）收集、培育、供应罗布麻素筛选植物材料；(4)开展大豆植物遗传学研究，阐明大豆生物活性物质水平变异的程度和遗传基础。植物学核心将识别并保存作为原始植物学获得的所有材料的代金券样本，包括由核心收集的植物标本代金券样本。接骨木和大豆的种质资源已经收集，从中将提供理想的化学成分的植物试验材料。将进行实地工作以获得更多的接骨木基因型，并将进行园艺研究以确定氮肥对化学成分的影响。将对可能含有罗布麻苷的北美本土植物进行调查，努力找到一种可靠的、可获得的、容易培养的化合物来源。一项大豆育种研究将确定与高水平和低水平感兴趣的化合物相关的基因型。这些研究将进一步表征研究项目所使用的测试材料，并加强对其结果的解释，使其相对于这些物种内更广泛的变异。此外，它们将促进为未来的研究选择高效材料的长期目标，包括临床试验，以及用于开发替代作物的优良基因型。

The Botanicals/Plant Genomics Core will support the Research Projects by supplying some botanical test materials, ensuring that identity and quality of botanical materials are properiy documented, and supplying samples for studies of infraspecific variation in species studied. Specific aims include (1) identifying, documenting, and supplying botanical raw materials; (2) collecting, cultivating, and supplying elderberry accessions for studies of infraspecific variation; (3) collecting, cultivating, and supplying plant materials for apocynin screening; and (4) conducting plant genetic studies in soybean to elucidate the extent and genetic basis of variation in the level of bioactive compounds. The Botanicals Core will identify and preserve voucher samples of all materials obtained as raw botanicals, including herbarium specimens to voucher samples collected by the Core. Elderberry and soy germplasm collections are already available, from which botanical test materials of desirable chemical composition will be supplied. Field work will be done to obtain more elderberry genotypes, and a horticultural study to determine the effect of nitrogen fertilization on chemical content will be performed. Native North American botanicals that might contain apocynin will be surveyed in an effort to locate a reliably obtainable, easily cultivated source of that compound. A soybean breeding study will identify genotypes associated with high and low levels of compounds of interest. These studies will further the characterization of test materials used by the Research Projects and enhance the interpretation of their results relative to the broader universe of variation within those species. Additionally, they will facilitate the longer-term goals of selecting high-potency materials for future studies, including clinical trials, and superior genotypes for use in developing alternative crops.