Botanical/Plant Genomics Core/Wendy Applequist

植物学/植物基因组学核心/Wendy Applequist

• 批准号: 8007170
• 负责人: DENNIS B LUBAHN
• 金额: $ 4.36万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007170
• 关键词: American; Analytical Chemistry; Area; Botanicals; Breeding; Chemicals; Clinical Trials; Collection; Development; Elderberry; Ensure; Fertilization; Fruit; Future; Genetic; Genistein; Genomics; Genotype; Goals; Guidelines; Human Resources; Isoflavones; Lead; Materials Testing; Methods; Monitor; Nitrogen; Phytosterols; Plants; Population; Production; Relative (related person); Research; Research Personnel; Research Project Grants; Sampling; Sampling Studies; Screening procedure; Services; Source; Soybeans; Specimen; Surveys; Variant; Work; acetovanillone; base; dietary supplements; interest; plant genetics; soy; voucher; working group

The Botanicals/Plant Genomics Core will support the Research Projects by supplying some botanical test materials, ensuring that identity and quality of botanical materials are properiy documented, and supplying samples for studies of infraspecific variation in species studied. Specific aims include (1) identifying, documenting, and supplying botanical raw materials; (2) collecting, cultivating, and supplying elderberry accessions for studies of infraspecific variation; (3) collecting, cultivating, and supplying plant materials for apocynin screening; and (4) conducting plant genetic studies in soybean to elucidate the extent and genetic basis of variation in the level of bioactive compounds. The Botanicals Core will identify and preserve voucher samples of all materials obtained as raw botanicals, including herbarium specimens to voucher samples collected by the Core. Elderberry and soy germplasm collections are already available, from which botanical test materials of desirable chemical composition will be supplied. Field work will be done to obtain more elderberry genotypes, and a horticultural study to determine the effect of nitrogen fertilization on chemical content will be performed. Native North American botanicals that might contain apocynin will be surveyed in an effort to locate a reliably obtainable, easily cultivated source of that compound. A soybean breeding study will identify genotypes associated with high and low levels of compounds of interest. These studies will further the characterization of test materials used by the Research Projects and enhance the interpretation of their results relative to the broader universe of variation within those species. Additionally, they will facilitate the longer-term goals of selecting high-potency materials for future studies, including clinical trials, and superior genotypes for use in developing alternative crops.

植物药/植物基因组学核心将通过提供一些植物测试材料来支持研究项目，确保植物材料的特性和质量得到适当记录，并为所研究物种的种下变异研究提供样本。具体目标包括（1）识别、记录和供应植物原料； (2) 收集、培育和提供接骨木浆果种质以用于种内变异的研究； （三）采集、栽培、供应用于罗布麻素筛选的植物材料； (4) 对大豆进行植物遗传学研究，以阐明生物活性化合物水平变异的程度和遗传基础。植物药核心将识别并保存作为原始植物药获得的所有材料的凭证样本，包括植物标本室标本，以凭证核心收集的样本。接骨木浆果和大豆种质资源已经可用，将从中提供具有所需化学成分的植物测试材料。将进行实地工作以获得更多接骨木果基因型，并将进行园艺研究以确定氮肥对化学含量的影响。我们将对可能含有罗布麻素的北美本土植物进行调查，以找到该化合物可靠且易于种植的来源。大豆育种研究将鉴定与高水平和低水平的目标化合物相关的基因型。这些研究将进一步确定研究项目所使用的测试材料的特征，并增强对其结果相对于这些物种内更广泛的变异的解释。此外，他们还将促进为未来研究（包括临床试验）选择高效材料以及用于开发替代作物的优良基因型的长期目标。