Botanical/Plant Genomics Core/Wendy Applequist

植物学/植物基因组学核心将通过提供一些植物学测试材料来支持研究项目，确保植物材料的身份和质量得到妥善记录，并为所研究物种的种下变异研究提供样本。具体目标包括：（1）鉴定、记录和提供植物原材料;（2）收集、培养和提供接骨木种质用于种下变异研究;（3）收集、培养和提供植物材料用于夹竹桃素筛选;（4）进行大豆植物遗传研究，以阐明生物活性化合物水平变异的程度和遗传基础。植物药核心将确定并保存作为原始植物药获得的所有材料的凭证样本，包括植物标本馆标本到核心收集的凭证样本。接骨木和大豆种质资源已经收集，从中将提供具有理想化学成分的植物试验材料。将进行田间工作，以获得更多的接骨木基因型，并进行园艺研究，以确定氮肥对化学含量的影响。可能含有夹竹桃素的北美本土植物将被调查，以努力找到一个可靠的，容易种植的该化合物的来源。一项大豆育种研究将确定与高水平和低水平感兴趣的化合物相关的基因型。这些研究将进一步表征研究项目所用的测试材料，并增强其结果相对于这些物种内更广泛的变异范围的解释。此外，它们将促进为未来研究（包括临床试验）选择高效材料和用于开发替代作物的上级基因型的长期目标。

The Botanicals/Plant Genomics Core will support the Research Projects by supplying some botanical test materials, ensuring that identity and quality of botanical materials are properiy documented, and supplying samples for studies of infraspecific variation in species studied. Specific aims include (1) identifying, documenting, and supplying botanical raw materials; (2) collecting, cultivating, and supplying elderberry accessions for studies of infraspecific variation; (3) collecting, cultivating, and supplying plant materials for apocynin screening; and (4) conducting plant genetic studies in soybean to elucidate the extent and genetic basis of variation in the level of bioactive compounds. The Botanicals Core will identify and preserve voucher samples of all materials obtained as raw botanicals, including herbarium specimens to voucher samples collected by the Core. Elderberry and soy germplasm collections are already available, from which botanical test materials of desirable chemical composition will be supplied. Field work will be done to obtain more elderberry genotypes, and a horticultural study to determine the effect of nitrogen fertilization on chemical content will be performed. Native North American botanicals that might contain apocynin will be surveyed in an effort to locate a reliably obtainable, easily cultivated source of that compound. A soybean breeding study will identify genotypes associated with high and low levels of compounds of interest. These studies will further the characterization of test materials used by the Research Projects and enhance the interpretation of their results relative to the broader universe of variation within those species. Additionally, they will facilitate the longer-term goals of selecting high-potency materials for future studies, including clinical trials, and superior genotypes for use in developing alternative crops.