Mu Center for Botanical Interaction Studies

穆植物相互作用研究中心

• 批准号: 8722446
• 负责人: DENNIS B LUBAHN
• 金额: $ 142.17万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8722446
• 关键词: AIDS/HIV problem; Acids; Address; Affect; American; Analytical Chemistry; Animal Model; Animal Nutrition; Animals; Antioxidants; Arts; Astrocytes; Bioinformatics; Biological; Biological Availability; Botanicals; Brain; Cell Culture Techniques; Cells; Cerebral Ischemia; Chemical Structure; Clinical; Clinical Sciences; Communicable Diseases; Complex Mixtures; Core Facility; DNA; Diet Research; Disease; Elderberry; Environment; Erinaceidae; Estrogens; Evaluation; Event; Faculty; Fostering; Funding; Genetic; Genomics; Goals; Grant; HIV; Health; Healthcare; Human; Immune; Individual; Infection; Inflammatory; Informatics; Infusion procedures; Institutes; Interdisciplinary Study; Intervention; Link; Listeriosis; Malignant Neoplasms; Malignant neoplasm of prostate; Measures; Messenger RNA; Methods; Mission; Missouri; Modeling; Molecular; Molecular Mechanisms of Action; NADPH Oxidase; NF-kappa B; Nerve Degeneration; Neurodegenerative Disorders; Neurons; Outcome; Pathway interactions; Phagocytes; Phytochemical; Phytoestrogens; Phytotherapy; Pilot Projects; Plants; Post-Translational Protein Processing; Principal Investigator; Property; Protein Analysis; Proteomics; Quality Control; Reactive Oxygen Species; Research; Research Personnel; Research Project Grants; Research Training; Resources; Role; Safety; Schools; Scientist; Sepsis; Services; Shapes; Signal Pathway; Signal Transduction; Stroke; Students; Technology; Testing; The Sun; Time; Tissues; Training; Training Support; Transgenic Mice; Translating; Translational Research; Translations; Universities; Veterinary Medicine; Work; antimicrobial; base; bench to bedside; career development; clinical practice; data integration; dietary supplements; experience; high risk; human disease; innovation; interest; metabolomics; new technology; novel; polyphenol; prevent; programs; protective effect; public health relevance; secondary infection; smoothened signaling pathway; soy; synergism

DESCRIPTION (provided by applicant): Botanicals are capable of interacting with several cell regulating pathways at the same time. Our Center's goal is to explore the crosstalk between antioxidant-related pathways. The 5 key pathways to be examined are the (anti)oxidant (Nrf2/Keap1 and NADPH oxidase) pathways and their interactions with the NF-kappaB-, estrogen-, and hedgehog-signaling pathways. Research Project 1: Botanicals Targeting 5 Signaling Pathways to Prevent Prostate Cancer will test the hypothesis that five individual, widely-used botanical dietary supplements will prevent prostate cancer through the five key signaling pathways. Research Proiect 2: Botanical Phenolics on Oxidative/Nitrosative Signaling Pathways: Implication for Cerebral Ischemia will test the hypothesis that botanicals promote brain health and prevent neurodegeneration through modulating the NADPH oxidase-, Nrf2/Keap1-, and/or NF-kappaB-signaling pathways, and protein modifications by S-nitrosylation. Research Project 3: Antioxidant Botanicals and Antimicrobial Defenses will test the hypothesis that botanical compounds that possess potent antioxidant activity modulate cellular events associated with antimicrobial defense. The Center includes five core facilities. The Administrative Core will oversee the Center's research, training, and pilot programs. Core A: Botanicals/Plant Genomics Core will identify, cultivate, and document raw plant materials including novel cultivars of elderberry and soy. Core B: Nutrition/Animal Core will provide transgenic mice and prepare diets for the research projects. Core C: Analytical Chemistry Core will provide analytical support and quality control for quantifying botanical compounds and their metabolites, and verifying plant material. Core D: Interactions Core: Mega-sequencing/Proteomics/lnformatics/Nitrosylation) will provide novel technology for proteomic and nitrosylation analyses, DNA mega-sequencing for mRNA analysis, and data integration through bioinformatics and statistical support. MU is highly supportive of this Center and is providing funds to train and support four graduate research assistants. Pilot projects will help provide seed money for new botanical grants and allow career development of both new faculty and students. Our Center's approach using bioinformatics to explore 5 signaling pathways concurrently by the latest cutting edge technology in mRNA and protein analysis is highly innovative. This type of synergism, when combined with the botanical and pathway expertise of Center investigators, will generate new information important in human health.

描述(申请人提供)：植物药能够同时与多个细胞调节途径相互作用。我们中心的目标是探索抗氧化剂相关途径之间的串扰。要研究的5个关键途径是(抗氧化)途径(Nrf2/Keap1和NADPH氧化酶)，以及它们与核因子-kappaB-、雌激素-和刺猬信号通路的相互作用。研究项目1：针对5个信号通路的植物制剂预防前列腺癌将测试5个单独的、广泛使用的植物膳食补充剂将通过5个关键信号通路预防前列腺癌的假设。研究项目2：植物酚对氧化/亚硝化信号通路的影响：对脑缺血的意义将检验这样一种假说，即植物通过调节NADPH氧化酶-、Nrf2/Keap1-和/或NF-kappaB-信号通路以及S-亚硝化修饰蛋白质来促进大脑健康和防止神经退行性变。研究项目3：抗氧化剂植物药和抗菌防御剂将测试具有强大抗氧化剂活性的植物化合物调节与抗菌防御剂相关的细胞事件的假设。该中心包括五个核心设施。行政核心将监督该中心的研究、培训和试点计划。核心A：植物学/植物基因组学核心将识别、培育和记录植物原材料，包括接骨木和大豆的新品种。核心B：营养/动物核心将提供转基因小鼠并为研究项目准备饲料。核心C：分析化学核心将为量化植物化合物及其代谢物和验证植物材料提供分析支持和质量控制。核心D：相互作用核心：Mega-sequencing/Proteomics/lnformatics/Nitrosylation)将为蛋白质组和亚硝化分析提供新技术，为信使核糖核酸分析提供DNA巨型测序，并通过生物信息学和统计支持进行数据整合。MU非常支持该中心，并提供资金培训和支持四名研究生研究助理。试点项目将有助于为新的植物学补助金提供种子资金，并允许新教师和新学生的职业发展。本中心采用生物信息学的方法，结合最新的先进的信使核糖核酸和蛋白质分析技术，同时探索5条信号通路，具有很强的创新性。这种类型的协同作用，当与中心研究人员的植物学和途径专业知识相结合时，将产生对人类健康重要的新信息。

项目成果

Fruit Composition of Elderberry (Sambucus spp.) Genotypes Grown in Oregon and Missouri, USA.

美国俄勒冈州和密苏里州种植的接骨木果基因型的果实成分。

• DOI: 10.17660/actahortic.2015.1061.24
• 发表时间: 2015
• 期刊: Acta horticulturae
• 作者: Perkins-Veazie,P;Thomas,AL;Byers,PL;Finn,CE
• 通讯作者: Finn,CE

From Gigabyte to Kilobyte: A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data.

• DOI: 10.1371/journal.pone.0125000
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Li J;Hou J;Sun L;Wilkins JM;Lu Y;Niederhuth CE;Merideth BR;Mawhinney TP;Mossine VV;Greenlief CM;Walker JC;Folk WR;Hannink M;Lubahn DB;Birchler JA;Cheng J
• 通讯作者: Cheng J

Allylation and Alkylation of Biologically Relevant Nucleophiles by Diallyl Sulfides.

• DOI: 10.1021/acs.joc.6b02517
• 发表时间: 2017-01
• 期刊: The Journal of organic chemistry
• 作者: Kasi Viswanatharaju Ruddraraju;Zachary D. Parsons;Calvin D. Lewis;K. Gates

Proteomic quantification and site-mapping of S-nitrosylated proteins using isobaric iodoTMT reagents.

• DOI: 10.1021/pr401179v
• 发表时间: 2014-07-03
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Qu, Zhe;Meng, Fanjun;Bomgarden, Ryan D.;Viner, Rosa I.;Li, Jilong;Rogers, John C.;Cheng, Jianlin;Greenlief, C. Michael;Cui, Jiankun;Lubahn, Dennis B.;Sun, Grace Y.;Gu, Zezong
• 通讯作者: Gu, Zezong

Inhibition of Gli/hedgehog signaling in prostate cancer cells by "cancer bush" Sutherlandia frutescens extract.

“癌丛”Sutherlandia frutescens 提取物抑制前列腺癌细胞中的 Gli/hedgehog 信号传导。

• DOI: 10.1002/cbin.10544
• 发表时间: 2016
• 期刊: Cell biology international
• 影响因子: 3.9
• 作者: Lin,Hui;Jackson,GlennA;Lu,Yuan;Drenkhahn,SaraK;Brownstein,KoreyJ;Starkey,NicholasJ;Lamberson,WilliamR;Fritsche,KevinL;Mossine,ValeriV;Besch-Williford,CynthiaL;Folk,WilliamR;Zhang,Yong;Lubahn,DennisB
• 通讯作者: Lubahn,DennisB