Mu Center for Botanical Interaction Studies

穆植物相互作用研究中心

• 批准号: 8509482
• 负责人: DENNIS B LUBAHN
• 金额: $ 11.36万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8509482
• 关键词: AIDS/HIV problem; Acids; Address; Affect; American; Analytical Chemistry; Animal Model; Animal Nutrition; Animals; Antioxidants; Arts; Astrocytes; Bioinformatics; Biological; Biological Availability; Botanicals; Brain; Cell Culture Techniques; Cells; Cerebral Ischemia; Chemical Structure; Clinical; Clinical Sciences; Communicable Diseases; Complex Mixtures; Core Facility; DNA; Diet Research; Disease; Elderberry; Environment; Erinaceidae; Estrogens; Evaluation; Event; Faculty; Fostering; Funding; Genetic; Genomics; Goals; Grant; HIV; Health; Healthcare; Human; Immune; Individual; Infection; Inflammatory; Informatics; Infusion procedures; Institutes; Interdisciplinary Study; Intervention; Link; Listeriosis; Malignant Neoplasms; Malignant neoplasm of prostate; Measures; Messenger RNA; Methods; Mission; Missouri; Modeling; Molecular; Molecular Mechanisms of Action; NADPH Oxidase; NF-kappa B; Nerve Degeneration; Neurodegenerative Disorders; Neurons; Outcome; Oxidants; Pathway interactions; Phagocytes; Phytochemical; Phytoestrogens; Phytotherapy; Pilot Projects; Plants; Post-Translational Protein Processing; Principal Investigator; Property; Protein Analysis; Proteomics; Quality Control; Reactive Oxygen Species; Research; Research Personnel; Research Project Grants; Research Training; Resources; Role; Safety; Schools; Scientist; Sepsis; Services; Shapes; Signal Pathway; Signal Transduction; Stroke; Students; Technology; Testing; The Sun; Time; Tissues; Training; Training Support; Transgenic Mice; Translating; Translational Research; Translations; Universities; Veterinary Medicine; Work; antimicrobial; base; bench to bedside; career development; clinical practice; data integration; dietary supplements; experience; high risk; human disease; innovation; interest; metabolomics; new technology; novel; polyphenol; prevent; programs; protective effect; public health relevance; secondary infection; smoothened signaling pathway; soy; synergism

DESCRIPTION (provided by applicant): Botanicals are capable of interacting with several cell regulating pathways at the same time. Our Center's goal is to explore the crosstalk between antioxidant-related pathways. The 5 key pathways to be examined are the (anti)oxidant (Nrf2/Keap1 and NADPH oxidase) pathways and their interactions with the NF-kappaB-, estrogen-, and hedgehog-signaling pathways. Research Project 1: Botanicals Targeting 5 Signaling Pathways to Prevent Prostate Cancer will test the hypothesis that five individual, widely-used botanical dietary supplements will prevent prostate cancer through the five key signaling pathways. Research Proiect 2: Botanical Phenolics on Oxidative/Nitrosative Signaling Pathways: Implication for Cerebral Ischemia will test the hypothesis that botanicals promote brain health and prevent neurodegeneration through modulating the NADPH oxidase-, Nrf2/Keap1-, and/or NF-kappaB-signaling pathways, and protein modifications by S-nitrosylation. Research Project 3: Antioxidant Botanicals and Antimicrobial Defenses will test the hypothesis that botanical compounds that possess potent antioxidant activity modulate cellular events associated with antimicrobial defense. The Center includes five core facilities. The Administrative Core will oversee the Center's research, training, and pilot programs. Core A: Botanicals/Plant Genomics Core will identify, cultivate, and document raw plant materials including novel cultivars of elderberry and soy. Core B: Nutrition/Animal Core will provide transgenic mice and prepare diets for the research projects. Core C: Analytical Chemistry Core will provide analytical support and quality control for quantifying botanical compounds and their metabolites, and verifying plant material. Core D: Interactions Core: Mega-sequencing/Proteomics/lnformatics/Nitrosylation) will provide novel technology for proteomic and nitrosylation analyses, DNA mega-sequencing for mRNA analysis, and data integration through bioinformatics and statistical support. MU is highly supportive of this Center and is providing funds to train and support four graduate research assistants. Pilot projects will help provide seed money for new botanical grants and allow career development of both new faculty and students. Our Center's approach using bioinformatics to explore 5 signaling pathways concurrently by the latest cutting edge technology in mRNA and protein analysis is highly innovative. This type of synergism, when combined with the botanical and pathway expertise of Center investigators, will generate new information important in human health. PUBLIC HEALTH RELEVANCE: The botanicals we propose to study are already extensively used by the public, and though they have antioxidant properties that may offer cancer, immuno-, and neuroprotective effects, the molecular basis of these effects is largely not understood. Our overall goal is to test and identify botanicals and related molecular mechanisms that can potentially offer beneficial effects to maintain a healthy body and brain by preventing neurodegenerative and infectious diseases, as well as cancer.

描述（由申请人提供）：植物药能够同时与几种细胞调节途径相互作用。我们中心的目标是探索抗氧化剂相关途径之间的串扰。要检查的5个关键途径是（抗）氧化剂（Nrf 2/Keap 1和NADPH氧化酶）途径及其与NF-κ B-、雌激素和hedgehog信号传导途径的相互作用。研究项目1：植物药靶向5个信号通路，以预防前列腺癌将测试的假设，五个个人，广泛使用的植物膳食补充剂将通过五个关键信号通路预防前列腺癌。研究项目二：植物酚类物质对氧化/亚硝化信号通路的影响：对脑缺血的影响将测试植物酚类物质通过调节NADPH氧化酶，Nrf 2/Keap 1和/或NF-κ B信号通路促进大脑健康和预防神经退行性变的假设，以及S-亚硝基化的蛋白质修饰。研究项目3：抗氧化植物药和抗菌防御将测试的假设，具有强大的抗氧化活性的植物化合物调节与抗菌防御相关的细胞事件。该中心包括五个核心设施。行政核心将监督中心的研究，培训和试点项目。核心A：植物学/植物基因组学核心将识别，培养和记录原植物材料，包括接骨木和大豆的新品种。核心B：营养/动物核心将提供转基因小鼠并为研究项目准备饮食。核心C：分析化学核心将为定量植物化合物及其代谢物提供分析支持和质量控制，并验证植物材料。核心D：互动核心：Mega-sequencing/Proteomics/Informatics/Nitrosylation）将为蛋白质组学和亚硝基化分析、用于mRNA分析的DNA Mega-sequencing以及通过生物信息学和统计支持的数据集成提供新技术。MU高度支持该中心，并提供资金培训和支持四名研究生研究助理。试点项目将有助于为新的植物学赠款提供种子资金，并允许新教师和学生的职业发展。本中心利用生物信息学的方法，通过mRNA和蛋白质分析的最新尖端技术同时探索5种信号通路，这是非常创新的。这种类型的协同作用，当与中心研究人员的植物学和途径专业知识相结合时，将产生对人类健康重要的新信息。 公共卫生关系：我们建议研究的植物药已经被公众广泛使用，尽管它们具有抗氧化特性，可能提供癌症，免疫和神经保护作用，但这些作用的分子基础在很大程度上还不清楚。我们的总体目标是测试和鉴定植物药和相关的分子机制，这些机制可以通过预防神经退行性疾病和传染病以及癌症来保持健康的身体和大脑。