IMOD SOFTWARE WORKSHOP

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 今年，我们实验室为来自全国和世界各地的14名参观者举办了一次关于断层扫描的研讨会。美国参与者包括来自伯纳姆研究所、哈佛大学、沃兹沃斯中心、亚利桑那州立大学、加州大学圣地亚哥分校、美国国立卫生研究院、加州大学戴维斯分校和布兰迪斯大学的人员。外国参观者包括来自英国曼彻斯特大学的参观者，来自不同马克斯·普朗克研究所的两名参观者，来自以色列魏兹曼研究所的一名参观者，来自赫尔辛基大学的一名参观者，以及来自乌得勒支的一名参观者。这些与会者听取了David Mastronarde关于断层摄影术理论和实践的讲座，并利用我们的图形用户界面eTomo，对IMOD的使用进行了广泛的动手指导。该实验室的几乎所有成员都提供了软件使用方面的指导，而且由于许多参与者都自带了高质量的笔记本电脑，因此每个人都有足够的计算设施来执行将一系列倾斜视图转换为3-D重建所涉及的每个过程。还为人们提供了使用显微镜体验连续EM和练习数据收集的时间。我们的参观者对这次研讨会的反应非常热烈，无论是当时填写的问卷还是参与者回到自己的实验室后发来的感谢信都表明了这一点。我们感到鼓舞的是，这种大约14名参观者的形式是我们可以很好地处理的，并通过它为将断层成像技术传播到各种其他实验室做出真正的贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This year, our laboratory hosted a workshop on tomography for 14 visitors from around the country and the world. American participants included people from the Burnham Institute, Harvard University, the Wadsworth Center, Arizona State University, University of California at San Diego, the National Institutes of Health, the University of California at Davis and Brandeis University. Foreign participants included visitors from the University of Manchester, England, two from different Max Planck Institutes, one from the Weizmann Institute in Israel, one from the University of Helsinki, and one from Utrecht. These participants heard lectures by David Mastronarde on the theory and practice of tomography, and they had extensive hands on instruction in the use of IMOD, taking advantage of our graphical user interface, eTomo. Instruction in software use was provided by essentially all members of the laboratory, and thanks to the fact that many participants brought their own high quality laptops, sufficient computational facilities were available for everyone to carry out each of the processes involved in converting a series of tilted views into a 3-D reconstruction. Time was also available for people to use the microscopes to experience serial EM and practice data collection. The responses of our visitors to this workshop were extremely enthusiastic, as indicated both by questionnaires filled out at the time and letters of thanks returned by the participants after they went back to their own laboratories. We are encouraged that this format of approximately 14 visitors is something we can handle well and through it make a real contribution to the dissemination of tomography technology to a variety of other labs.