IMOD SOFTWARE WORKSHOP

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 今年，我们的实验室为来自全国和世界各地的 14 名参观者举办了断层扫描研讨会。 美国参与者包括来自伯纳姆研究所、哈佛大学、沃兹沃斯中心、亚利桑那州立大学、加州大学圣地亚哥分校、国立卫生研究院、加州大学戴维斯分校和布兰代斯大学的人员。 外国参与者包括来自英国曼彻斯特大学的访客、来自不同马克斯·普朗克研究所的两名访客、一名来自以色列魏茨曼研究所的访客、一名来自赫尔辛基大学的访客和一名来自乌得勒支的访客。 这些参与者听取了 David Mastronarde 关于断层扫描理论和实践的讲座，并利用我们的图形用户界面 eTomo 获得了有关 IMOD 使用的大量实践指导。 软件使用指导基本上由实验室的所有成员提供，并且由于许多参与者都带来了自己的高质量笔记本电脑，因此每个人都可以使用足够的计算设施来执行将一系列倾斜视图转换为 3D 重建所涉及的每个过程。 人们还有时间使用显微镜体验串行电镜并练习数据收集。 从当时填写的调查问卷以及参与者回到自己的实验室后寄回的感谢信都可以看出，我们的参观者对本次研讨会的反应非常热情。 令我们感到鼓舞的是，我们能够很好地处理这种大约 14 名参观者的形式，并通过它为向其他各种实验室传播断层扫描技术做出真正的贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year, our laboratory hosted a workshop on tomography for 14 visitors from around the country and the world. American participants included people from the Burnham Institute, Harvard University, the Wadsworth Center, Arizona State University, University of California at San Diego, the National Institutes of Health, the University of California at Davis and Brandeis University. Foreign participants included visitors from the University of Manchester, England, two from different Max Planck Institutes, one from the Weizmann Institute in Israel, one from the University of Helsinki, and one from Utrecht. These participants heard lectures by David Mastronarde on the theory and practice of tomography, and they had extensive hands on instruction in the use of IMOD, taking advantage of our graphical user interface, eTomo. Instruction in software use was provided by essentially all members of the laboratory, and thanks to the fact that many participants brought their own high quality laptops, sufficient computational facilities were available for everyone to carry out each of the processes involved in converting a series of tilted views into a 3-D reconstruction. Time was also available for people to use the microscopes to experience serial EM and practice data collection. The responses of our visitors to this workshop were extremely enthusiastic, as indicated both by questionnaires filled out at the time and letters of thanks returned by the participants after they went back to their own laboratories. We are encouraged that this format of approximately 14 visitors is something we can handle well and through it make a real contribution to the dissemination of tomography technology to a variety of other labs.