IMOD SOFTWARE WORKSHOP

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 今年，我们的实验室为来自全国各地和世界各地的14位参观者举办了一个关于断层扫描的研讨会。 美国的参与者包括来自伯纳姆研究所、哈佛大学、沃兹沃斯中心、亚利桑那州立大学、加州大学圣地亚哥分校、美国国立卫生研究院、加州大学戴维斯分校和布兰迪斯大学的人。 外国与会者包括来自英国曼彻斯特大学的访问者，两名来自不同的马克斯·普朗克研究所，一名来自以色列魏茨曼研究所，一名来自赫尔辛基大学，一名来自乌得勒支。 这些参与者听取了大卫Mastronarde关于断层扫描理论和实践的讲座，并利用我们的图形用户界面eTomo，对IMOD的使用进行了广泛的指导。 基本上实验室的所有成员都提供了软件使用的指导，并且由于许多参与者都带了自己的高质量笔记本电脑，因此每个人都有足够的计算设施来执行将一系列倾斜视图转换为3D重建的过程。 人们也有时间使用显微镜体验连续EM和实践数据收集。 我们的访问者对这次研讨会的反应非常热烈，无论是当时填写的问卷调查还是与会者回到自己的实验室后回复的感谢信都表明了这一点。 我们感到鼓舞的是，这种大约14名参观者的形式是我们可以很好地处理的，并通过它为向其他各种实验室传播断层扫描技术做出真实的贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year, our laboratory hosted a workshop on tomography for 14 visitors from around the country and the world. American participants included people from the Burnham Institute, Harvard University, the Wadsworth Center, Arizona State University, University of California at San Diego, the National Institutes of Health, the University of California at Davis and Brandeis University. Foreign participants included visitors from the University of Manchester, England, two from different Max Planck Institutes, one from the Weizmann Institute in Israel, one from the University of Helsinki, and one from Utrecht. These participants heard lectures by David Mastronarde on the theory and practice of tomography, and they had extensive hands on instruction in the use of IMOD, taking advantage of our graphical user interface, eTomo. Instruction in software use was provided by essentially all members of the laboratory, and thanks to the fact that many participants brought their own high quality laptops, sufficient computational facilities were available for everyone to carry out each of the processes involved in converting a series of tilted views into a 3-D reconstruction. Time was also available for people to use the microscopes to experience serial EM and practice data collection. The responses of our visitors to this workshop were extremely enthusiastic, as indicated both by questionnaires filled out at the time and letters of thanks returned by the participants after they went back to their own laboratories. We are encouraged that this format of approximately 14 visitors is something we can handle well and through it make a real contribution to the dissemination of tomography technology to a variety of other labs.