WORKSHOP FOR TRAINING MICROSCOPE ADMINISTRATORS IN THE SETUP AND USE OF SERIALEM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 今年，我们增加了一个研讨会，面向显微镜管理员设置和使用SerialEM。研讨会于2006年12月6日至8日举行，我们接待了来自以下机构的6名参与者：卡尔加里大学、贝勒医学院、德克萨斯大学健康科学中心、纽约结构生物学中心、布兰迪斯大学和魏茨曼研究所。参与者的数量被故意保持在较少的水平，主要是为了让每个参与者有足够的时间真正学习如何在显微镜上使用SerialEM。另外，我们只能在每个显微镜房间里舒适地多放3个人。工作坊的设计是上午授课，下午有密集的实践经验。第一天的重点是介绍SerialEM并为TILT系列自动收购做好准备。第二天的重点是低剂量的应用，包括冷冻电子断层扫描、能量过滤和导航器。最后，第三天集中讨论了宏的使用、SerialEM的校准和数码相机。每个人都接受了电子和纸质形式的讲座和讲义，这样他们就可以使用这些工具，培训他们的显微镜设备用户如何使用SerialEM。所有参与者都给予了好评。此外，与会者还就如何改进SerialEM获得了宝贵的反馈。三名参与者接受了额外的培训；一名接受了高倍率断层扫描，两名接受了低温倾斜序列的重建。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year, we added an additional workshop that targeted microscope administrators for the setup and use of SerialEM. The workshop was held December 6-8, 2006 where we hosted 6 participants from the following institutions: University of Calgary, Baylor College of Medicine, University of Texas Health Science Center, New York Structural Biology Center, Brandeis University, and The Weizmann Institute. The number of participants was kept deliberately small mainly to give each participant enough time to really learn how to use SerialEM on the microscopes. In addition, we could only put 3 additional people in each microscope room comfortably. The workshop was designed to have a morning lecture and an afternoon with intensive hands-on experience. The first day focused on introducing SerialEM and setting up for automated tilt-series acquisition. The second day focused on low-dose applicationsincluding cryo-electron tomography, energy filtering, and the Navigator. Finally, the third day focused on using macros, calibration of SerialEM, and digital cameras. Each person received the lectures and handouts in both electronic and paper format so they could use those tools train their microscope facility users on how to use SerialEM. All participants gave favorable evaluations. In addition, valuable feedback was obtained from the participants on how to improve SerialEM. Three participants received extra training; one for high magnification tomography, and two for reconstruction of cryo tilt-series.