WORKSHOP FOR TRAINING MICROSCOPE ADMINISTRATORS IN THE SETUP AND USE OF SERIALEM

培训显微镜管理员设置和使用 Serialem 的研讨会

• 批准号: 7955050
• 负责人: DAVID N MASTRONARDE
• 金额: $ 2.14万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955050
• 关键词: Administrator; Calibration; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Dose; Educational workshop; Electronics; Evaluation; Feedback; Funding; Grant; Hand; Health Sciences; Institutes; Institution; Learning; Medicine; Microscope; New York; Paper; Participant; Persons; Research; Research Personnel; Resources; Series; Source; Texas; Time; Training; United States National Institutes of Health; Universities; college; design; digital; electron tomography; experience; improved; lectures; reconstruction; structural biology; tomography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year, we added an additional workshop that targeted microscope administrators for the setup and use of SerialEM. The workshop was held December 6-8, 2006 where we hosted 6 participants from the following institutions: University of Calgary, Baylor College of Medicine, University of Texas Health Science Center, New York Structural Biology Center, Brandeis University, and The Weizmann Institute. The number of participants was kept deliberately small mainly to give each participant enough time to really learn how to use SerialEM on the microscopes. In addition, we could only put 3 additional people in each microscope room comfortably. The workshop was designed to have a morning lecture and an afternoon with intensive hands-on experience. The first day focused on introducing SerialEM and setting up for automated tilt-series acquisition. The second day focused on low-dose applicationsincluding cryo-electron tomography, energy filtering, and the Navigator. Finally, the third day focused on using macros, calibration of SerialEM, and digital cameras. Each person received the lectures and handouts in both electronic and paper format so they could use those tools train their microscope facility users on how to use SerialEM. All participants gave favorable evaluations. In addition, valuable feedback was obtained from the participants on how to improve SerialEM. Three participants received extra training; one for high magnification tomography, and two for reconstruction of cryo tilt-series.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 今年，我们增加了一个额外的研讨会，针对显微镜管理员设置和使用 SerialEM。该研讨会于 2006 年 12 月 6 日至 8 日举行，我们接待了来自以下机构的 6 名参与者：卡尔加里大学、贝勒医学院、德克萨斯大学健康科学中心、纽约结构生物学中心、布兰迪斯大学和魏茨曼研究所。参与者的数量故意保持较小，主要是为了让每个参与者有足够的时间来真正学习如何在显微镜上使用 SerialEM。此外，每个显微镜室只能舒适地容纳另外 3 个人。该研讨会设计为上午的讲座和下午的密集实践经验。第一天的重点是介绍 SerialEM 并设置自动倾斜系列采集。第二天的重点是低剂量应用，包括冷冻电子断层扫描、能量过滤和导航器。最后，第三天的重点是使用宏、SerialEM 的校准和数码相机。每个人都收到电子和纸质格式的讲座和讲义，以便他们可以使用这些工具培训显微镜设施用户如何使用 SerialEM。所有参与者都给出了积极的评价。此外，还从参与者那里获得了关于如何改进 SerialEM 的宝贵反馈。三名参与者接受了额外培训；一个用于高倍断层扫描，两个用于重建低温倾斜系列。