MICROTUBULE DYNAMICS IN YEAST

酵母中的微管动力学

• 批准号: 8362540
• 负责人: David J. Odde
• 金额: $ 1.06万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362540
• 关键词: Behavior; Cell physiology; Cells; Cellular Structures; Collaborations; Data; Data Set; Funding; Grant; Kinesin; Kinetochores; Laboratories; Lead; Life; Manuscripts; Metaphase; Microtomy; Microtubules; Minnesota; Mitotic spindle; Modeling; Motor; National Center for Research Resources; North Carolina; Principal Investigator; Regulation; Research; Research Infrastructure; Resources; Saccharomyces cerevisiae; Source; Structure; Testing; United States National Institutes of Health; Work; Yeasts; base; cellular imaging; cost; interest; mathematical model; mutant; reconstruction; three dimensional structure; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Odde lab (Univ. Minnesota) is interested in understanding the mitotic spindle in yeast at the level of interplay of motor function and microtubule dynamics such that the can mathematical model mitotic spindles. Their initial modeling work is presented in (Gardner M.K. et al. (2005) Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. Mol Biol Cell. 16:3764-3775.). To build this mathematical model they used previous 3D ultrastructural data on yeast mitotic spindles from the Boulder Laboratory (Winey, M., et al. (1995) Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol. 129:1601-1616.). To expand their modeling and to test the model by using it to predict the behavior of spindles in yeast strains mutant in various spindle components, Dave Odde has arranged a collaboration between his group (mathematical modeling) and that of Kerry Bloom (Univ. North Carolina - Chapel Hill, live-cell imaging) and Mark Winey and the Boulder Lab for the 3D Structure of Cells (EM tomography). Thus far, we have revisited wild-type spindle structure using tomography to confirm the previous results based on reconstruction from serial thin sections and to serve as controls for the analysis of mutants. We have also been collecting datasets and modeling spindles from a mutant strain lacking the CIN8 kinesin-like motor, which are very interesting. This work is proving very fruitful and may lead to a manuscript this year.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 奇数实验室(大学明尼苏达州)感兴趣的是在运动功能和微管动力学相互作用的水平上了解酵母中的有丝分裂纺锤体，从而可以建立有丝分裂纺锤体的数学模型。他们最初的建模工作在(Gardner M.K.等人)中介绍。(2005)动粒微管动力学的张力依赖调节可以解释酵母中的中期聚集。摩尔生物电池。16：3764-3775。)。为了建立这个数学模型，他们使用了来自Boulder实验室的关于酵母有丝分裂纺锤体的先前3D超微结构数据(Winey，M.，et al.(1995)酿酒酵母有丝分裂纺锤体的三维超微结构分析。J.细胞生物学。129：1601-1616。为了扩展他们的建模并通过使用该模型来预测在各种纺锤体组件中突变的酵母菌株的纺锤体行为来测试该模型，Dave Odde安排了他的团队(数学建模)和Kerry Bloom(大学)的合作。北卡罗来纳州-教堂山，活细胞成像)和马克·维尼和博尔德实验室的细胞三维结构(EM断层扫描)。到目前为止，我们已经使用断层摄影术重新观察了野生型纺锤体结构，以证实基于连续薄片重建的先前结果，并作为突变分析的对照。我们还一直在收集数据集，并对缺乏CIN8动蛋白样马达的突变株的纺锤体进行建模，这非常有趣。这项工作被证明是非常有成效的，可能会在今年产生一份手稿。