GINS MOTION REVEALS REPLICATION FORK PROGRESSION IS REMARKABLY UNIFORM
GINS MOTION 揭示复制叉进展非常均匀
基本信息
- 批准号:8361518
- 负责人:
- 金额:$ 5.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-03-01 至 2012-03-31
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAffectArchitectureBiologicalBiological AssayChromatin StructureComplexDNADNA biosynthesisDataDoctor of MedicineEpigenetic ProcessFrequenciesFundingFungal GenomeGenetic TranscriptionGenomeGenomicsGrantLocationMaintenanceManuscriptsMicroarray AnalysisModelingMotionMovementNational Center for Research ResourcesPrincipal InvestigatorProductionPublishingResearchResearch InfrastructureResourcesSaccharomycetalesSimulateSourceSystemTimeUnited States National Institutes of HealthYeastschromatin immunoprecipitationcostinsightmacromoleculemembernovelrepairedresearch study
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Previous studies have led to a picture wherein the replication of DNA progresses at variable rates over different parts of the budding yeast genome. These prior experiments, focused on production of nascent DNA, have been interpreted to imply that the dynamics of replication fork progression are strongly affected by local chromatin structure/architecture, and by interaction with machineries controlling transcription, repair and epigenetic maintenance. Here, we adopted a complementary approach for assaying replication dynamics using whole genome time-resolved chromatin immunoprecipitation combined with microarray analysis of the GINS complex, an integral member of the replication fork. Surprisingly, our data show that this complex progresses at highly uniform rates regardless of genomic location, revealing that replication fork dynamics in yeast is simpler and more uniform than previously envisaged. In addition, we show how the synergistic use of experiment and modeling leads to novel biological insights. In particular, a parsimonious model allowed us to accurately simulate fork movement throughout the genome and also revealed a subtle phenomenon, which we interpret as arising from low-frequency fork arrest.
A manuscript describing these results has been published:
M.D. Sekedat, D. Feny¿, R.S. Rogers, A.J. Tackett, J.D. Aitchison, B.T. Chait "GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome" Mol Systems Biol, 6 (2010) 353
这个子项目是许多利用资源的研究子项目之一
由NIH/NCRR资助的中心拨款提供。子项目的主要支持
而子项目的主要调查员可能是由其他来源提供的,
包括其它NIH来源。 列出的子项目总成本可能
代表子项目使用的中心基础设施的估计数量,
而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。
先前的研究已经导致了这样一幅图景,其中DNA的复制在芽殖酵母基因组的不同部分以可变的速率进行。这些先前的实验,专注于新生DNA的产生,已被解释为暗示复制叉进展的动力学受到局部染色质结构/架构的强烈影响,以及与控制转录、修复和表观遗传维持的机制的相互作用。在这里,我们采用了一种互补的方法来测定复制动态使用全基因组时间分辨染色质免疫沉淀结合微阵列分析的GINS复合物,复制叉的一个组成部分。令人惊讶的是,我们的数据表明,无论基因组位置如何,这种复合体都以高度均匀的速率进展,这表明酵母中的复制叉动态比之前设想的更简单、更均匀。此外,我们展示了如何协同使用实验和建模导致新的生物学见解。特别是,一个简约的模型使我们能够准确地模拟整个基因组的叉运动,也揭示了一个微妙的现象,我们解释为低频叉逮捕所产生的。
描述这些结果的手稿已出版:
M.D. Sekedat,D. Feny?,R.S.放大图片作者:A. Aitchison,B.T. Chait“GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome”Mol Systems Biol,6(2010)353
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MATTHEW SEKEDAT其他文献
MATTHEW SEKEDAT的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MATTHEW SEKEDAT', 18)}}的其他基金
ON THE ROLE OF THE GINS COMPLEX IN REPLICATION
关于杜松子酒复合物在复制中的作用
- 批准号:
8169138 - 财政年份:2010
- 资助金额:
$ 5.22万 - 项目类别:
ON THE ROLE OF THE GINS COMPLEX IN REPLICATION
关于杜松子酒复合物在复制中的作用
- 批准号:
7954098 - 财政年份:2009
- 资助金额:
$ 5.22万 - 项目类别:
ON THE ROLE OF THE GINS COMPLEX IN REPLICATION
关于杜松子酒复合物在复制中的作用
- 批准号:
7722244 - 财政年份:2008
- 资助金额:
$ 5.22万 - 项目类别:
ON THE ROLE OF THE GINS COMPLEX IN REPLICATION
关于杜松子酒复合物在复制中的作用
- 批准号:
7355146 - 财政年份:2006
- 资助金额:
$ 5.22万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 5.22万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 5.22万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 5.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 5.22万 - 项目类别:
Studentship