DETECTION AND CORRECTION OF INTERFERENCE IN MRM ANALYSIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 多反应监测(MRM)是在复杂背景下准确定量低丰度蛋白质的一种选择方法。然而，低丰度蛋白质的MRM分析对样品中其他组分的干扰很敏感，这些组分具有与监测的转变相同的前体和片段质量。在MRM分析的开发过程中，通常要注意避免这种干扰，然而，当完成的分析应用于真实样品时，仍可能出现意想不到的干扰。我们使用计算机模拟作为工具来研究在MRM分析中对干扰进行校正的可行性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Multiple Reaction Monitoring (MRM) is a method of choice for accurate quantitation of low-abundance proteins in a complex background. MRM analysis of low abundance proteins is, however, sensitive to interference from other components in the sample that have the same precursor and fragment masses as the monitored transitions. Care is usually taken to avoid such interferences during development of MRM assays, however, unanticipated interference may still appear when the finished assay is applied to real samples. We used computer simulations as a tool to investigate the feasibility of correction for interference in MRM analyses.