PROTEIN QUANTITATION USING MASS SPECTROMETRY

使用质谱法进行蛋白质定量

• 批准号: 8361557
• 负责人: David Fenyo
• 金额: $ 1.04万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361557
• 关键词: Area; Biology; Computing Methodologies; Funding; Grant; Mass Spectrum Analysis; Measures; Methods; National Center for Research Resources; Paper; Peptides; Principal Investigator; Proteins; Publishing; Research; Research Infrastructure; Resources; Sampling; Source; United States National Institutes of Health; Work; cost; interest; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mass spectrometry is a method of choice for quantifying low abundance proteins and peptides and it has been applied to many areas of biology. We are developing a range of computational methods for protein and peptide quantification, including methods for finding and integrating peaks of interest, and detecting interference to obtain a robust measure of the amount of material present in the samples. A paper describing this work has been published: Zhang G, Ueberheide BM, Waldemarson S, Myung S, Molloy K, Eriksson J, Chait BT, Neubert TA, Feny¿ D Protein quantitation using mass spectrometry Methods Mol Biol. 2010;673:211-22

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 质谱法是一种定量低丰度蛋白质和肽的首选方法，已应用于生物学的许多领域。 我们正在开发一系列用于蛋白质和肽定量的计算方法，包括用于寻找和整合感兴趣的峰的方法，以及检测干扰以获得样品中存在的物质的量的稳健测量。已经发表了一篇描述这项工作的论文： Zhang G，Ueberheide BM，Waldemarson S，Myung S，Molloy K，Eriksson J，Chait BT，Neubert TA，Feny D Protein quantitation using mass spectrometry Methods Mol Biol.2010;673：211-22