STATISTICAL BASIS FOR DETERMINING SIGNIFICANCE OF LOCALIZATION OF MODIFICATIONS

确定修改本地化重要性的统计基础

• 批准号: 8361583
• 负责人: David Fenyo
• 金额: $ 0.26万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361583
• 关键词: Cell physiology; Funding; Grant; Location; Mass Spectrum Analysis; Methods; Modification; National Center for Research Resources; Post-Translational Modification Site; Post-Translational Protein Processing; Principal Investigator; Process; Research; Research Infrastructure; Resources; Risk; Source; United States National Institutes of Health; base; cost; macromolecule; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Post-translational modifications of proteins are critical for regulating cellular processes and our understanding of these processes relies on the ability to determine the precise location of these modifications. Tandem mass spectrometry is potentially well suited for the determination of post-translational modification sites. Several methods have been proposed to determine the most likely location for a modification given a tandem mass spectrum, but currently there is no framework for judging a localization assignment in terms of a statistical significance level  i.e., the risk that a localization assignment has occurred by chance.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质的翻译后修饰对于调节细胞过程至关重要，我们对这些过程的理解依赖于确定这些修饰的精确位置的能力。串联质谱法可能非常适合于翻译后修饰位点的测定。已经提出了几种方法来确定给定串联质谱的修饰的最可能位置，但是目前没有根据统计显著性水平来判断定位分配的框架  也就是说，本地化分配偶然发生的风险。