ROS Analytical Core
ROS 分析核心
基本信息
- 批准号:7795675
- 负责人:
- 金额:$ 12.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:Cell membraneCellsChemicalsCollaborationsCore FacilityGenerationsHistopathologyImageLifeLipid PeroxidationLipidsMeasurementMeasuresModelingOxidative StressPainPathogenesisPeripheralPhospholipidsProteinsReactive Oxygen SpeciesRoleSourceTimeUnsaturated Fatty AcidsWorkcell fixingcellular imagingcentral sensitizationfunctional outcomesin vivooxidationperoxidation
项目摘要
Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects
require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging,
particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known
propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress,
however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell
membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the
functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid
peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying
mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by
the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C.
Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS
generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned
Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology
branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS
levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the
imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of
Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core
becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in
pain.
核心B是ROS分析核心。这是一个必要的新核心,因为所有的项目
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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BHUPENDRA S KAPHALIA其他文献
BHUPENDRA S KAPHALIA的其他文献
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{{ truncateString('BHUPENDRA S KAPHALIA', 18)}}的其他基金
AMPKa agonist in attenuating CPT1A inhibition and alcoholic chronic pancreatitis
AMPKa 激动剂减轻 CPT1A 抑制和酒精性慢性胰腺炎
- 批准号:
10649275 - 财政年份:2023
- 资助金额:
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Role of Alcohol Metabolism in Alcoholic Chronic Pancreatitis
酒精代谢在酒精性慢性胰腺炎中的作用
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9897449 - 财政年份:2017
- 资助金额:
$ 12.75万 - 项目类别:
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脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
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6546600 - 财政年份:2002
- 资助金额:
$ 12.75万 - 项目类别:
Fatty Acid Ethyl Esters in Ethanol-induced Pancreatitis
脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
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7103385 - 财政年份:2002
- 资助金额:
$ 12.75万 - 项目类别:
Fatty Acid Ethyl Esters in Ethanol-induced Pancreatitis
脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
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7184042 - 财政年份:2002
- 资助金额:
$ 12.75万 - 项目类别:
Fatty Acid Ethyl Esters in Ethanol-induced Pancreatitis
脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
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6619844 - 财政年份:2002
- 资助金额:
$ 12.75万 - 项目类别:
Fatty Acid Ethyl Esters in Ethanol-induced Pancreatitis
脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
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- 资助金额:
$ 12.75万 - 项目类别:
Fatty Acid Ethyl Esters in Ethanol-induced Pancreatitis
脂肪酸乙酯在乙醇诱发的胰腺炎中的作用
- 批准号:
6778274 - 财政年份:2002
- 资助金额:
$ 12.75万 - 项目类别:
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