ROS Analytical Core

核心B是ROS分析核心。这是一个新的核心，因为所有的项目， 需要定量测量活性氧（ROS）。测量ROS是一项挑战， 特别是当在体内进行时，由于细胞中ROS的多种细胞内来源和已知的 一种形式的活性氧容易转化为另一种形式的倾向。氧化应激的一个重要方面， 然而，是细胞中中性脂质和磷脂不饱和脂肪酸部分的过氧化 膜和细胞蛋白质的氧化。此外，测量特定的ROS可能无法解释 病理性疼痛和相关发病机制的功能结果。ROS诱导的脂质 蛋白质的过氧化和氧化似乎是理解潜在的 我们的疼痛模型。脂质过氧化和氧化蛋白的发现将得到以下结果的支持： 实时活细胞和组织化学固定细胞成像将与核心C合作进行。 因此，ROS Analytical Core将测量脂质过氧化产物和氧化蛋白， 在活细胞或固定细胞中产生。ROS分析核心由三个分支组成， 各项目的联合总监：1）脂质过氧化分析，2）蛋白质氧化分析，和3）组织病理学 树枝分支1）和2）用于化学分析，分支3）用于测量细胞内ROS 使用实时活细胞成像和来自固定细胞的组织化学成像两者的水平。的使用 成像设施，这个核心与核心C（成像核心）和联合主任非常密切地合作， 这个核心的历史学也是成像核心的联合主任。因此，ROS分析核心 成为PPG的一个组成部分，用于描述ROS在中枢和外周致敏中的作用， 痛苦

Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging, particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress, however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C. Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in pain.